PXD005220-1

PXD005220 is an original dataset announced via ProteomeXchange.

Title	Proteomic profiles of synovial fluids derived from autologous chondrocyte implantation surgery
Description	The pathology of failure in currently used biological treatments (cell therapies and microfracture) for cartilage repair or early osteoarthritis (OA) is poorly understood. We aimed to identify a reliable panel of biological predictors, present in synovial fluid, which can be used in the preoperative setting to optimise patient selection and therapy efficacy. The-long term aim is to reduce the number of patients who progress to end-stage OA and require knee replacement by making earlier, less invasive treatments more effective. In this first stage, we have taken synovial fluid aspirates from patients undergoing autologous chondrocyte implantation (ACI) at our centre in Oswestry, UK. Synovial fluids (SFs) were obtained from 14 ACI responders (mean Lysholm improvement of 33 (17-54)) and 13 non-responders (mean Lysholm decrease of 14 (4-46)) at the two stages of surgery (cartilage harvest and chondrocyte implantation). Dynamic range compression of synovial fluids coupled with label-free quantification mass spectrometry (MS), was used in an unbiased approach to identify predictive markers of ACI success or failure and to investigate the biological pathways involved in the clinical response to ACI.
HostingRepository	PRIDE
AnnounceDate	2017-07-04
AnnouncementXML	Submission_2017-07-04_00:05:50.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD005220
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Emma Wilson
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Oxidation; Carbamidomethyl
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-10-25 01:32:01	ID requested
⏵ 1	2017-07-04 00:05:51	announced

Hulme CH, Wilson EL, Peffers MJ, Roberts S, Simpson DM, Richardson JB, Gallacher P, Wright KT, Autologous chondrocyte implantation-derived synovial fluids display distinct responder and non-responder proteomic profiles. Arthritis Res Ther, 19(1):150(2017) [pubmed]

curator keyword: Biomedical

submitter keyword: Label-free

synovial fluid

proteominer

osteoarthritis

cell therapy

Mandy Peffers
contact affiliation	University of Liverpool
contact email	peffs@liverpool.ac.uk
lab head
Emma Wilson
contact affiliation	University of Chester
contact email	e.wilson@chester.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD005220
     1. Label: PRIDE project
     2. Name: Proteomic profiles of synovial fluids derived from autologous chondrocyte implantation surgery