PXD005207 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | PfCDPK1 mediated signaling in erythrocytic stages of Plasmodium falciparum |
Description | Calcium Dependent Protein Kinases (CDPKs) are key effectors of calcium signaling in malaria parasite. We have elucidated a novel role of PfCDPK1, which is indispensable for the erythrocytic development of Plasmodium falciparum, in invasion of host Red Blood Cells (RBCs). To gain insights into the mechanisms via which PfCDPK1 is involved in parasitic processes, a comparative phosphoproteomic analysis was employed to identify its parasitic targets. Proteins were isolated from CDPK1 knockdown and wild type parasites and processed for trypsin digestion and iTRAQ labeling. Quantitative proteomic analysis using high resolution mass spectrometry led to the identification of hypophosphorylated proteins, which can be the likely substrates for this kinase. In addition, in vitro kinase assays and antibody based validation of the hypophosphorylated sites was carried out. This study will provide a novel insight into the CDPK1 mediated invasion by Plasmodium falciparum. |
HostingRepository | PRIDE |
AnnounceDate | 2017-05-02 |
AnnouncementXML | Submission_2017-09-13_00:09:25.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Keshava Prasad T. S. |
SpeciesList | scientific name: Plasmodium falciparum; NCBI TaxID: 5833; |
ModificationList | iTRAQ4plex-116 reporter+balance reagent acylated residue; phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-10-24 02:01:17 | ID requested | |
1 | 2017-05-02 05:15:46 | announced | |
⏵ 2 | 2017-09-13 00:09:26 | announced | Updated publication reference for PubMed record(s): 28680058. |
Publication List
Kumar S, Kumar M, Ekka R, Dvorin JD, Paul AS, Madugundu AK, Gilberger T, Gowda H, Duraisingh MT, Keshava Prasad TS, Sharma P, PfCDPK1 mediated signaling in erythrocytic stages of Plasmodium falciparum. Nat Commun, 8(1):63(2017) [pubmed] |
Keyword List
curator keyword: Biological, Biomedical |
submitter keyword: Orbitrap Fusion, Phosphoproteome |
Contact List
Keshava Prasad |
contact affiliation | Faculty Scientist, Institute of Bioinformatics, Bangalore, India |
contact email | keshav@ibioinformatics.org |
lab head | |
Keshava Prasad T. S. |
contact affiliation | Institute of Bioinformatics, Bangalore, Karnataka, India. |
contact email | keshav@ibioinformatics.org |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD005207
- Label: PRIDE project
- Name: PfCDPK1 mediated signaling in erythrocytic stages of Plasmodium falciparum