PXD005203-1

PXD005203 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Staphylococcus aureus Secretome Profiling using Label Free Quantitation
Description	The application of label free quantitation (LFQ) to the profiling of secreted proteomes is a powerful tool in the deciphering of these mechanisms and the determination of the severity of infection with different strains. Our study consists of three distinct, but interconnected experiments resulting in an extensive data set for the identification of antigens. First we explore the changes in virulence factor secretion when regulators are mutated, then compare these effects in two different growth conditions. We further explore the effects of nutrient accessibility by profiling the secreted proteomes of S. aureus at different points in the colony growth curve in both a rich and minimal media. Lastly, the secreted proteins from a selection of twelve strain types representing 5 colony complexes are quantified and compared to the cytotoxicity of the strains. Biological triplicates were used for mutant and growth curve studies.
HostingRepository	MassIVE
AnnounceDate	2017-02-14
AnnouncementXML	Submission_2017-02-14_05:29:20.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jessica Chapman
SpeciesList	scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationList	No PTMs are included in the dataset
Instrument	instrument model: Fusion Lumos Tribrid with ETD

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2016-10-21 07:04:17	ID requested
⏵ 1	2017-02-14 05:29:20	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: secretome, label free quantitation

submitter keyword: secretome, label free quantitation

Contact List

principal_investigator
lab head
Jessica Chapman
contact affiliation	NYU School of Medicine
contact email	jessica.chapman@nyumc.org
dataset submitter

Full Dataset Link List

MassIVE dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000080260

MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000080260