PXD005176-1

PXD005176 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomic comparison of SW480 and SW620
Description	Quantitative phosphoproteome analyses of colorectal cancer cell lines (metastatis: SW620, non-metastatis: SW480) were carried out using IMAC-based posphopeptide enrichment followed by SCX-RP-MS/MS with SILAC labeling (SW480: light-label, SW620: heavy-label). The obtained results were searched against UniProt DB by Mascot, and the identified peptides were filtered at 1% FDR thresholds by searching against the randomized decoy DB at peptide level. This dataset with the PX complete submission format will be further used to generate a customized DB in this jPOST project.
HostingRepository	jPOST
AnnounceDate	2016-10-18
AnnouncementXML	Submission_2016-10-17_21:29:09.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mariko Ito
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue; (2S,3R)-3-hydroxyaspartic acid; L-aspartic 4-phosphoric anhydride; 6x(13)C labeled residue; 6x(13)C,4x(15)N labeled L-arginine
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-10-17 10:36:10	ID requested
⏵ 1	2016-10-17 21:29:09	announced
2	2016-11-17 18:14:34	announced	Updated keyword list.
3	2017-03-30 23:38:19	announced	Updated Repository support.
4	2017-03-31 00:18:32	announced	Updated DigitalObjectIdentifier.
5	2018-09-18 23:46:13	announced	Updated CV, FTP location and Modification list.
6	2021-02-04 17:15:06	announced	2021-02-04: Updated FTP location.
7	2022-09-18 03:04:04	announced	2022-09-18: Updated FTP location.

Dataset with its publication pending

submitter keyword: Chromosome-Centric Human Proteome Project

colorectal cancer

FASP

IMAC

missing protein

neXtProt

phosphopeptide

PhosphoSitePlus

Takeshi Tomonaga
lab head
Mariko Ito
contact affiliation	Kyoto Univ.
dataset submitter

jPOST dataset URI