PXD005174-1

PXD005174 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomic data of HCT116 cells
Description	Quantitative phosphoproteome analyses of colorectal cancer cell lines (HCT116 metastatic clone and the parent clone without metastasis) were carried out using IMAC-based posphopeptide enrichment followed by SCX-RP-MS/MS with iTRAQ labeling(metastasis:iTRAQ116 or 117, no metastasis: iTRAQ114 or 115). The obtained results were searched against UniProt DB by Mascot, and the identified peptides were filtered at 1% FDR thresholds by searching against the randomized decoy DB at peptide level. This dataset with the PX complete submission format will be further used to generate a customized DB in this jPOST project.
HostingRepository	jPOST
AnnounceDate	2016-10-18
AnnouncementXML	Submission_2016-10-17_21:28:38.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mariko Ito
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue; (2S,3R)-3-hydroxyaspartic acid; Applied Biosystems iTRAQ(TM) multiplexed quantitation chemistry; iTRAQ4plex; L-aspartic 4-phosphoric anhydride
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-10-17 08:15:21	ID requested
⏵ 1	2016-10-17 21:28:39	announced
2	2017-03-30 23:37:44	announced	Updated Repository support.
3	2017-03-31 00:16:48	announced	Updated DigitalObjectIdentifier.
4	2018-09-18 23:45:11	announced	Updated CV, FTP location and Modification list.
5	2021-02-04 17:16:10	announced	2021-02-04: Updated FTP location.
6	2022-09-18 03:11:31	announced	2022-09-18: Updated FTP location.

Dataset with its publication pending

submitter keyword: Chromosome-Centric Human Proteome Project

colorectal cancer

FASP

IMAC

missing protein

neXtProt

phosphopeptide

PhosphoSitePlus

Takeshi Tomonaga
lab head
Mariko Ito
contact affiliation	Kyoto Univ.
dataset submitter

jPOST dataset URI