PXD005155-1

PXD005155 is an original dataset announced via ProteomeXchange.

Title	Mapping protein interactions of sodium channel NaV1.7 using epitope-tagged gene targeted mice
Description	The voltage-gated sodium channel NaV1.7 plays a critical role in pain pathways. As well as action potential propagation, NaV1.7 regulates neurotransmitter release, integrates depolarizing inputs over long periods and regulates transcription. In order to better understand these functions, we generated an epitope-tagged NaV1.7 mouse that showed normal NaV1.7 channel activity and normal pain behavior. Analysis of NaV1.7 complexes affinity-purified under native conditions by mass spectrometry revealed 267 NaV1.7 associated proteins including known and novel interactors such as sodium channel β3 subunit (Scn3b) and collapsin response mediator protein (Crmp2). Selected NaV1.7 protein interactors, such as Crmp2, membrane-trafficking protein synapototagmin-2 (Syt2), G protein-regulated inducer of neurite outgrowth 1 (Gprin1), L-type amino acid transporter 1 (Lat1) and transmembrane P24 trafficking protein 10 (Tmed10) were validated using co-immunoprecipitation and functional assays. The information provided with this physiologically normal epitope-tagged mouse should provide useful insights into the downstream mechanisms associated with NaV1.7 channel function.
HostingRepository	PRIDE
AnnounceDate	2018-01-15
AnnouncementXML	Submission_2018-01-15_07:21:27.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	honglei huang
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-10-14 06:37:15	ID requested
⏵ 1	2018-01-15 07:21:28	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: The voltage-gated sodium channel NaV1.7, epitope-tagged NaV1.7 mouse, pain, mice, NaV1.7 complexes affinity-purification, LC-MS/MS

Benedikt Kessler
contact affiliation	Mass Spectrometry Laboratory, Target Discovery Institute, University of Oxford, The Old Road Campus, Oxford, OX3 7FZ, UK
contact email	benedikt.kessler@ndm.ox.ac.uk
lab head
honglei huang
contact affiliation	NDM, University of oxford
contact email	honglei.huang@ndm.ox.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/01/PXD005155

PRIDE project URI

• PRIDE
  1. PXD005155
     1. Label: PRIDE project
     2. Name: Mapping protein interactions of sodium channel NaV1.7 using epitope-tagged gene targeted mice