PXD005101-1

PXD005101 is an original dataset announced via ProteomeXchange.

Title	Lipid metabolism modulation is associated with molecular injury and kidney dysfunction in a rat ischemia model
Description	Due to organ shortage, the transplantation community are increasingly using kidney from deceased donors such as donation after circulatory death (DCD) and donation after brain death (DBD). However, DCD donation have increased delayed graft function compared to DBD, an underline mechanism is still not well defied at the molecular level. In this study, we employed a rat model to mimic warm ischemia (45 mins) and reperfusion injury (IRI) for 4h and 24h. Apoptotic and tissue histological staining confirmed apoptosis and necrosis occurred at 4h and 24h post IRI respectively. Tissue proteome study revealed acute phase response, coagulation and complement activation and lipid X receptor activation as major pathway altered in intervention kidneys. Metabolomics follow up disclosed an increased level of lipids and fatty acids (FA). Mitochondrial function analysed by mitochondrial complex I activity and oxygen consumption and ATP levels in intervention kidney tissues were maintained 4h post IRI, but was significantly reduced 24h post IRI. Integrated proteo-metabolome analysis discovered an increased FA beta-oxidation 4h post IRI to sustain energy production. Kidney function were declined 24h post IRI indicated by increased blood creatinine and lactate levels. This study provides the frame work for the design of future metabolic intervention strategies to minimize kidney injury.
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_10:06:04.545.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD005101
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Philip Charles
SpeciesList	scientific name: Rattus rattus (Black rat); NCBI TaxID: 10117;
ModificationList	Oxidation
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2016-10-05 03:33:03	ID requested
⏵ 1	2024-10-07 10:06:09	announced

10.6019/PXD005101;

Huang H, van Dullemen LFA, Akhtar MZ, Faro ML, Yu Z, Valli A, Dona A, Th, é, z, é, nas ML, Charles PD, Fischer R, Kaisar M, Leuvenink HGD, Ploeg RJ, Kessler BM, Proteo-metabolomics reveals compensation between ischemic and non-injured contralateral kidneys after reperfusion. Sci Rep, 8(1):8539(2018) [pubmed]

10.1038/s41598-018-26804-8;

curator keyword: Biological

submitter keyword: Metabolomics, Lipid Metabolism,β-oxidation, Kidney Transplantation, Ischaemia Reperfusion Injury (IRI), Proteomics, Fatty Acids, GCXGC-MS

Benedikt Kessler
contact affiliation	Target Discovery Institute, NDMRB, University of Oxford
contact email	benedikt.kessler@ndm.ox.ac.uk
lab head
Philip Charles
contact affiliation	University of Oxford
contact email	philip.charles@ndm.ox.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD005101
     1. Label: PRIDE project
     2. Name: Lipid metabolism modulation is associated with molecular injury and kidney dysfunction in a rat ischemia model