PXD005096-2

PXD005096 is an original dataset announced via ProteomeXchange.

Title	Microproteomics application for the limited number of cells
Description	Mass spectrometry (MS)-based proteomics explores in-depth proteome, however, the analysis of cell subsets, which might be minor population, is hampered due to a multistep procedure for sample preparation and purification. Along those lines, optimization of proteomics workflow toward limited number of cells is an asset. Here, we report our workflow of microproteomics, proteomics for a limited number of cells: a microfluidic-chip based cell sorter was applied for a damage-free cell sorting, and each 100 or 1000 THP-1 cells were collected in hydrophilic or albumin-coated microtubes. Following in-solution enzymatic digestions, peptide mixtures were purified and applied to nanoLC-MS (Orbitrap Elite) analysis with 3 hr gradient. Our method enabled proteome mapping from 1000-sorted cells, with a similar in-depth map from 1 μg of cell lysate. This approach would be useful for cell subsets and limited materials such as circulating tumor cells, inflammatory cells in cancer tissues and clinical materials.
HostingRepository	jPOST
AnnounceDate	2017-07-18
AnnouncementXML	Submission_2017-07-31_00:41:18.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kie Kasuga
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue; N6-acetyl-L-lysine; unknown modification: Carbamylation(N-terminal); unknown modification: Gln-> pyro-Glu (N-terminal); unknown modification: Carbamidomethyl(N-term); (2S,3R)-3-hydroxyaspartic acid; unknown modification: deamidation(NQ); unknown modification: Carbamylation (N-term); unknown modification: Glu->pyro-Glu (N-Term); unknown modification: Carbamidomethylation (N-term); unknown modification: deamidation (NQ)
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2016-10-04 22:54:26	ID requested
1	2017-07-18 06:53:51	announced
⏵ 2	2017-07-31 00:41:19	announced	Updated PubMed.
3	2017-07-31 00:44:31	announced	Updated PubMed.
4	2018-09-18 22:56:23	announced	Updated CV, FTP location and Modification list.
5	2021-02-04 16:44:24	announced	2021-02-04: Updated FTP location.
6	2022-09-18 03:22:52	announced	2022-09-18: Updated FTP location.

submitter keyword: THP-1, shotgun proteomics, Cell-sorting, microproteomics

Kie Kasuga
lab head
Kie Kasuga
contact affiliation	Kanazawa University
dataset submitter

jPOST dataset URI