PXD005058 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomics exploiting modular baits with different subcellular locations reveal hydrogen peroxide-inducible clone 5 protein (HIC-5) and a glucocorticoid receptor variant as novel testin partners |
Description | The LIM domain and tumour suppressor protein Testin (Tes) is downregulated in a variety of human tumours and tumour cell lines. Depending on its conformation, Tes localises to stress fibres and focal adhesions where it forms protein complexes with other members of the actin cytoskeleton, such as zyxin and VASP, and thereby influences cellular processes like cell migration, adhesion or spreading. Tes is a modular protein and Tes variants lacking specific domains, localize to different locations in cells. To better understand the molecular basis of its function, we utilized an interaction proteomics approach combined with pathway analysis. This revealed proteins present in complexes in which Tes participates as a function of its modular structure as well as novel Tes interaction partners. We demonstrate that Tes interacts with a short isoform of the glucocorticoid receptor (GR), independently of the conventional full length GRĪ±, a transcription factor important in regulating cellular metabolism and immune function. Tes also interacts with the focal adhesion protein and GR-coactivator Hic-5. In addition, we found that upon overexpression, Tes and Hic-5 induce opposite effects on cell spreading on a fibronectin matrix. |
HostingRepository | PRIDE |
AnnounceDate | 2018-10-26 |
AnnouncementXML | Submission_2018-10-26_13:37:08.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | An Staes |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-09-28 08:48:13 | ID requested | |
⏵ 1 | 2018-10-26 13:37:09 | announced | |
Publication List
Sala S, Catillon M, Hadzic E, Schaffner-Reckinger E, Van Troys M, Ampe C, The PET and LIM1-2 domains of testin contribute to intramolecular and homodimeric interactions. PLoS One, 12(5):e0177879(2017) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: Testin,IP,Proteomics, LC-MS/MS, iMixPro, GR, HIC-5 |
Contact List
Christophe Ampe |
contact affiliation | Department of Biochemistry, Ghent University, Belgium |
contact email | christophe.ampe@ugent.be |
lab head | |
An Staes |
contact affiliation | Medical Protein Chemistry |
contact email | an.staes@vib-ugent.be |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD005058 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD005058
- Label: PRIDE project
- Name: Proteomics exploiting modular baits with different subcellular locations reveal hydrogen peroxide-inducible clone 5 protein (HIC-5) and a glucocorticoid receptor variant as novel testin partners