PXD005045-1

PXD005045 is an original dataset announced via ProteomeXchange.

Title	Comparative proteome of the BeWo and HTR8/SVneo trophoblastic cell lines
Description	Here, we have undertaken comparative label-free quantitative (LFQ) proteomic profiling of the proteins expressed in BeWo and HTR8/SVneo cell lines using a mass spectrometry approach. We have identified 1557 proteins in total. 915 proteins were expressed in both cell lines including various transcription factors, chaperones and transport proteins. A further 338 and 304 proteins were uniquely expressed in BeWo and HTR8/SVneo cells respectively. Our bioinformatics analysis reflects the known epithelial and mesenchymal phenotypes of these cell models with principal differences in GO observed in ‘Cell junction’, ‘Catenin complex’ as well as ‘Cell adhesion’ and ‘Cell differentiation’. Our novel comparative proteomic analysis of these trophoblastic cell lines will set the stage for the use of trophoblastic cell lines for the study of pregnancy disorders and further research focusing on placental function.
HostingRepository	PRIDE
AnnounceDate	2018-07-05
AnnouncementXML	Submission_2018-07-05_05:44:05.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Paulina Szklanna
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-09-27 04:17:25	ID requested
⏵ 1	2018-07-05 05:44:06	announced
2	2024-10-22 04:32:48	announced	2024-10-22: Updated project metadata.

Szklanna PB, Wynne K, Nolan M, Egan K, Á, inle FN, Maguire PB, Comparative proteomic analysis of trophoblast cell models reveals their differential phenotypes, potential uses, and limitations. Proteomics, 17(10):e1700037(2017) [pubmed]

curator keyword: Biological

submitter keyword: Comparative proteomics, label-free quantitation, trophoblastic cell lines.

Patricia B. Maguire
contact affiliation	University College Dublin
contact email	patricia.maguire@ucd.ie
lab head
Paulina Szklanna
contact affiliation	University College Dublin
contact email	paulina.szklanna@ucdconnect.ie
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/07/PXD005045

PRIDE project URI

• PRIDE
  1. PXD005045
     1. Label: PRIDE project
     2. Name: Comparative proteome of the BeWo and HTR8/SVneo trophoblastic cell lines