PXD005025-2

PXD005025 is an original dataset announced via ProteomeXchange.

Title	Label-free Proteomics Reveals that Cowpea Severe Mosaic Virus Transiently Suppresses the Host Leaf Protein Accumulation During the Compatible Interaction with Cowpea (Vigna unguiculata [L.] Walp.)
Description	Viruses are important plant pathogens that threaten diverse crops worldwide. Diseases caused by Cowpea severe mosaic virus (CPSMV) have drawn attention because of the serious damages they cause to economically important crops including cowpea. This work was undertaken to quantify and identify the responsive proteins of a susceptible cowpea genotype infected with CPSMV, in comparison with mock-inoculated controls, using label-free quantitative proteomics and databanks, aiming at providing insights on the molecular basis of this compatible interaction. Cowpea leaves were mock-inoculated or inoculated with CPSMV and 2 and 6 days later proteins were extracted and analyzed. More than 3000 proteins were identified and 75 and 55 of them differentially accumulated in response to CPSMV, at 2 and 6 DAI, respectively. At 2 DAI, 76% of the proteins were down-represented and 24% upaccumulated. However, at 6 DAI, 100% of the identified proteins were up-accumulated. Thus CPSMV transiently suppresses the synthesis of proteins involved particularly in the redox homeostasis, protein synthesis, defense, stress, RNA/DNA metabolism, signaling, and other functions, allowing viral invasion and spread in cowpea tissues. It is expected that identification of differentially accumulated proteins and their interactions advance our understanding on how a susceptible cowpea genotype responds to CPSMV infection.
HostingRepository	PRIDE
AnnounceDate	2017-10-24
AnnouncementXML	Submission_2017-10-24_03:02:14.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gustavo De Souza
SpeciesList	scientific name: Vigna mungo (Rice bean) (Black gram); NCBI TaxID: 3915;
ModificationList	monohydroxylated residue; iodoacetic acid derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-09-22 08:31:37	ID requested
1	2017-01-02 12:57:32	announced
⏵ 2	2017-10-24 03:02:15	announced	Updated project metadata.
3	2024-10-22 04:32:44	announced	2024-10-22: Updated project metadata.

Paiva AL, Oliveira JT, de Souza GA, Vasconcelos IM, Label-free Proteomic Reveals that Cowpea Severe Mosaic Virus Transiently Suppresses the Host Leaf Protein Accumulation During the Compatible Interaction with Cowpea (Vigna unguiculata [L.] Walp.). J Proteome Res, 15(12):4208-4220(2016) [pubmed]

curator keyword: Biological

submitter keyword: Vigna unguiculata, CPSMV, compatible interaction, Label-free proteomic

Luiza Paiva
contact affiliation	Laboratory of Plant Defense Proteins, Department of Biochemistry and Molecular Biology, Federal University of Ceara, Fortaleza, Brazil.
contact email	luizapaiva.lp@hotmail.com
lab head
Gustavo De Souza
contact affiliation	Brain Institute, Federal University of Rio Grande do Norte
contact email	gadsouza@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD005025
     1. Label: PRIDE project
     2. Name: Label-free Proteomics Reveals that Cowpea Severe Mosaic Virus Transiently Suppresses the Host Leaf Protein Accumulation During the Compatible Interaction with Cowpea (Vigna unguiculata [L.] Walp.)