PXD005012-1

PXD005012 is an original dataset announced via ProteomeXchange.

Title	Identifying SAM-binding proteins in D.melanogaster
Description	The aim of the project is to identify proteins the ustilize S-adenosyl methionine as cofactor for performing methylation of target molecules in D. melanogaster S2 cells. For this purpose, we applied a small-molecule capturing protocol using a SAH-capture compound with UV-activated crosslinker and biotin immobilization groups. We tested the protein affinity with and without crosslinking for two different photo-activatable crosslinkers benzophenone and nitrobenzene, respectively, as well as different concentrations of the capture compound. As control we incubated the cell lysate with the streptavidin beads without adding the capture compound. Two specific capture compounds containing a S-adenosyl homocysteine as capturing functionality and either benzophenone or azo-benzene as reactive crosslinkers as well as a biotin group for affinity purification were tested in this protocol. One sample representing a HeLa cell lysate (Waters) under equal conditions was added to the dataset.
HostingRepository	PRIDE
AnnounceDate	2019-03-11
AnnouncementXML	Submission_2019-03-11_05:51:30.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andreas Schmidt
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2016-09-21 04:23:41	ID requested
⏵ 1	2019-03-11 05:51:30	announced
2	2024-10-22 04:33:55	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

curator keyword: Biological

submitter keyword: S-Adenosyl methionine, methylation, capture compound, fragmentation

Axel Imhof
contact affiliation	Protein Analysis Unit ZfP LMU Munich, BioMedical Center Grosshaderner Strasse 9 82152 Planegg-Martinsried Tel. +49 89 2180 71804
contact email	imhof@lmu.de
lab head
Andreas Schmidt
contact affiliation	Adolf-Butenandt-Institut
contact email	A.Schmidt@med.uni-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/03/PXD005012

PRIDE project URI

• PRIDE
  1. PXD005012
     1. Label: PRIDE project
     2. Name: Identifying SAM-binding proteins in D.melanogaster