PXD004934-1

PXD004934 is an original dataset announced via ProteomeXchange.

Title	Identification of novel RHOA interacting patners
Description	RHO subfamily of small GTPases comprise highly conserved family members RHOA, RHOB, and RHOC which cycle between GTP-bound 'active' and GDP-bound 'inactive' states. In the active form, RHO proteins interact with a variety of downstream effector proteins, controlling their activity and function. Many of the RHO subfamily effector proteins such as ROCK, PKN, and mDIA, are key regulators of actin cytoskeleton and cell motility. To identify novel effector proteins for RHOA,we carried out a GST-pulldown from heavy or light SILAC labelled HeLa cells using GST tagged GTP-bound RHOA, or GST alone control, as bait. Pulldowns were performed in duplicates with switched labellings. Specific interactors were destinguished from the background on the basis of SILAC Heavy to Light ratios between GST-RHOA and GST alone pulldowns.
HostingRepository	PRIDE
AnnounceDate	2016-11-01
AnnouncementXML	Submission_2016-11-01_03:22:19.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Faraz Mardakheh
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-09-12 02:55:12	ID requested
⏵ 1	2016-11-01 03:22:20	announced
2	2024-10-22 04:31:47	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

curator keyword: Biological

submitter keyword: SILAC

LC-MS

protein-protein interactions

RHOA

Christopher J Marshall
contact affiliation	Division of Cancer Biology, Institute of Cancer Research, 237 Fulham Road, London SW3 6JB, The United Kingdom.
contact email	chris.marshall@icr.ac.uk
lab head
Faraz Mardakheh
contact affiliation	Institute of Cancer Research
contact email	MARDAKHEH@ICR.AC.UK
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/11/PXD004934

PRIDE project URI

• PRIDE
  1. PXD004934
     1. Label: PRIDE project
     2. Name: Identification of novel RHOA interacting patners