PXD004922 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Matrisome remodelling during Intervertebral Disc development and ageing |
Description | Intervertebral disc (IVD) is often the cause of low back pain. Degeneration occurs with age and is accompanied by extracellular matrix (ECM) depletion, culminating in nucleus pulpous (NP) extrusion and IVD destruction. Although the changes that occur with ageing in the IVD have been under study, not much attention has been given to ECM remodelling during normal development. Therefore, a thorough study of ECM composition and a comprehensive view on how this microenvironment is affected in normal ageing conditions is needed, to better understand the processes involved in IVD development and in age-associated degeneration. We have compared the NP matrisome of bovine IVDs from foetus, young and old animals by quantitative iTRAQ LC-MS/MS. Protein expression levels of the most interesting candidates were validated by Western Blot analysis. In total, 161 bovine proteins were identified. Of these, 77 molecules were common to the three different age groups, of which 36 defined the NP matrisome. Differential expression levels obtained for Collagen Type XI, XII, XIV, Fibronectin and Prolargin were independently confirmed. Herein, we demonstrate a shift in NP matrisome signatures that occurs in healthy bovine IVDs with development and ageing. Thus, this study provides insight into factors that may explain age-associated IVD degeneration, and which are putative targets for therapy. It also highlights key molecules, characteristic of early developmental stages, which constitute potential effectors in IVD regeneration. |
HostingRepository | PRIDE |
AnnounceDate | 2017-09-25 |
AnnouncementXML | Submission_2017-09-25_02:07:36.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Cátia Santa |
SpeciesList | scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; |
ModificationList | No PTMs are included in the dataset |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-09-12 01:53:12 | ID requested | |
⏵ 1 | 2017-09-25 02:07:38 | announced | |
Publication List
Caldeira J, Santa C, Os, ó, rio H, Molinos M, Manadas B, Gon, ç, alves R, Barbosa M, Matrisome Profiling During Intervertebral Disc Development And Ageing. Sci Rep, 7(1):11629(2017) [pubmed] |
Keyword List
curator keyword: Biological, Biomedical |
submitter keyword: Intervertebral Disc (IVD) |
Extracellular Matrix (ECM) |
Nucleus Pulpous (NP) |
iTRAQ |
Contact List
Bruno Manadas |
contact affiliation | Center for Neuroscience and Cell Biology |
contact email | bmanada@gmail.com |
lab head | |
Cátia Santa |
contact affiliation | Center for Neuroscience and Cell Biology |
contact email | catiajmsanta@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD004922
- Label: PRIDE project
- Name: Matrisome remodelling during Intervertebral Disc development and ageing