PXD004904-2

PXD004904 is an original dataset announced via ProteomeXchange.

Title	Rapid High-pH Reverse Phase StageTip for Sensitive Small-Scale Membrane Proteomic Profiling
Description	To increase the coverage of membrane protein identification,we developed high-pH reverse phase (Hp-RP) combined with stop-and-go extraction tip (StageTip) technique, as a fast (∼15 min.). Almost 5000 proteins (∼60% membrane proteins) can be identified in only 10 μg of membrane protein digests, showing the superior sensitivity of the Hp-RP StageTip approach.
HostingRepository	jPOST
AnnounceDate	2016-09-08
AnnouncementXML	Submission_2016-11-17_18:22:38.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pei-Yi Lin
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue; N6-acetyl-L-lysine; (2S,3R)-3-hydroxyaspartic acid
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2016-09-07 23:59:47	ID requested
1	2016-09-07 23:59:49	announced
⏵ 2	2016-11-17 18:22:40	announced	Updated publication list.
3	2018-09-18 23:23:28	announced	Updated CV, FTP location and Modification list.
4	2021-02-04 17:07:07	announced	2021-02-04: Updated FTP location.
5	2022-09-18 03:40:59	announced	2022-09-18: Updated FTP location.

Dimayacyac-Esleta BR, Tsai CF, Kitata RB, Lin PY, Choong WK, Lin TD, Wang YT, Weng SH, Yang PC, Arco SD, Sung TY, Chen YJ, Rapid High-pH Reverse Phase StageTip for Sensitive Small-Scale Membrane Proteomic Profiling. Anal Chem, 87(24):12016-23(2015) [pubmed]

submitter keyword: Membrane Proteomic, Rapid High-pH Reverse Phase StageTip

Baby Rorielyn T. Dimayacyac-Esleta and Yu-Ju Chen
lab head
Pei-Yi Lin
contact affiliation	Academia Sinica
dataset submitter

jPOST dataset URI