PXD004876 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Characterization of genetic loss-of-function of Fus in zebrafish |
Description | The RNA-binding protein FUS is implicated in transcription, alternative splicing of neuronal genes and DNA repair. Mutations in FUS have been linked to human neurodegenerative diseases such as ALS (amyotrophic lateral sclerosis). We genetically disrupted fus in zebrafish (Danio rerio) using the CRISPR-Cas9 system. The fus knockout animals are fertile and did not show any distinctive phenotype. Mutation of fus induces mild changes in gene expression on the transcriptome and proteome level in the adult brain. We observed a significant influence of genetic background on gene expression and 3’UTR usage, which could mask the effects of loss of Fus. Unlike published fus morphants, maternal zygotic fus mutants do not show motoneuronal degeneration and exhibit normal locomotor activity. |
HostingRepository | PRIDE |
AnnounceDate | 2018-06-15 |
AnnouncementXML | Submission_2018-06-15_05:44:58.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | F Butter |
SpeciesList | scientific name: Danio rerio (Zebrafish) (Brachydanio rerio); NCBI TaxID: 7955; |
ModificationList | monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-08-19 05:47:57 | ID requested | |
⏵ 1 | 2018-06-15 05:44:59 | announced | |
Publication List
Lebedeva S, de Jesus Domingues AM, Butter F, Ketting RF, Characterization of genetic loss-of-function of Fus in zebrafish. RNA Biol, 14(1):29-35(2017) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: zebrafish, fus protein, CRISPR |
Contact List
Falk Butter |
contact affiliation | Institute of Molecular Biology (IMB) |
contact email | f.butter@imb-mainz.de |
lab head | |
F Butter |
contact affiliation | Dept. of Proteomics and Signal Transduction |
contact email | f.butter@imb-mainz.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD004876 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD004876
- Label: PRIDE project
- Name: Characterization of genetic loss-of-function of Fus in zebrafish