PXD004875-2

PXD004875 is an original dataset announced via ProteomeXchange.

Title	Quantitative Chemical Proteomic Profiling of Ubiquitin Specific Proteases in Intact Cancer Cells
Description	Deubiquitinating enzymes play an important role in a plethora of therapeutically relevant processes, and are emerging as pioneering drug targets. Herein, we present a novel Ubiquitin Specific Protease (USP) inhibitor, alongside an alkyne-tagged activity-based probe analogue. Activity-based proteome profiling identified 12 USPs, including USP4, USP16, and USP33, as inhibitor targets using nanomolar probe concentrations. This represents the first intact cell activity-based profiling of deubiquitinating enzymes. Further analysis demonstrated functional inhibition of USP33 and identified a synergistic relationship in combination with ATR inhibition, consistent with USP4 inhibition.
HostingRepository	PRIDE
AnnounceDate	2017-10-24
AnnouncementXML	Submission_2017-10-24_03:03:04.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jenny Ward
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	6x(13)C labeled residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-08-19 03:41:40	ID requested
1	2016-12-21 01:09:15	announced
⏵ 2	2017-10-24 03:03:05	announced	Updated project metadata.

Ward JA, McLellan L, Stockley M, Gibson KR, Whitlock GA, Knights C, Harrigan JA, Jacq X, Tate EW, Quantitative Chemical Proteomic Profiling of Ubiquitin Specific Proteases in Intact Cancer Cells. ACS Chem Biol, 11(12):3268-3272(2016) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: LC-MSMS, Chemical proteomics, Ubiquitin, ABPP

Edward W Tate
contact affiliation	Department of Chemistry, Imperial College London, London, SW7 2AZ, United Kingdom.
contact email	e.tate@imperial.ac.uk
lab head
Jenny Ward
contact affiliation	Imperial College London
contact email	j.ward11@imperial.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD004875
     1. Label: PRIDE project
     2. Name: Quantitative Chemical Proteomic Profiling of Ubiquitin Specific Proteases in Intact Cancer Cells