PXD004868-1

PXD004868 is an original dataset announced via ProteomeXchange.

Title	Altered signaling associated with chronic arsenic exposure in human skin keratinocytes
Description	Modulation of signaling pathways upon chronic arsenic exposure remains poorly studied. Here we carried out SILAC-based quantitative phosphoproteomics analysis to dissect the signaling induced upon chronic arsenic exposure in human skin keratinocyte cell line, HaCaT. We identified 4,171 unique phosphosites derived from 2,000 proteins. We observed differential phosphorylation of 406 phosphosites (2-fold) corresponding to 305 proteins. Several pathways involved in cytoskeleton maintenance and organization were found to be significantly enriched (p<0.05). Our data revealed altered phosphorylation of proteins associated with adherens junction remodeling and actin polymerization. Kinases such as protein kinase C iota type (PRKCI), mitogen-activated protein kinase kinase kinase 1 (MAP3K1), tyrosine-protein kinase BAZ1B (BAZ1B) and STE20 like kinase (SLK) were found to be hyperphosphorylated. Our study provides novel insights into signaling perturbations associated with chronic arsenic exposure in human skin keratinocytes.
HostingRepository	PRIDE
AnnounceDate	2017-08-11
AnnouncementXML	Submission_2017-08-11_04:03:53.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Harsha Gowda
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; 6x(13)C labeled residue; monohydroxylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-08-19 01:47:50	ID requested
⏵ 1	2017-08-11 04:03:55	announced
2	2024-10-22 04:32:31	announced	2024-10-22: Updated project metadata.

Mir SA, Renuse S, Sathe G, Khan AA, Patil AH, Nanjappa V, Bhat FA, Prasad TSK, Giri AK, Chatterjee A, Gowda H, Altered signaling associated with chronic arsenic exposure in human skin keratinocytes. Proteomics Clin Appl, 11(11-12):(2017) [pubmed]

submitter keyword: HaCaT, SILAC, Arsenic, Chronic exposure

Harsha Gowda
contact affiliation	Institute of Bioinformatics, Bangalore, India
contact email	harsha@ibioinformatics.org
lab head
Harsha Gowda
contact affiliation	Institute of Bioinformatics
contact email	harsha@ibioinformatics.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/08/PXD004868

PRIDE project URI

• PRIDE
  1. PXD004868
     1. Label: PRIDE project
     2. Name: Altered signaling associated with chronic arsenic exposure in human skin keratinocytes