PXD004857-2

PXD004857 is an original dataset announced via ProteomeXchange.

Title	Effect of oncogenic transformation on metabolism (synthetic peptides)
Description	The expression levels of metabolic enzymes were estimated by MRM analysis with internal standard recombinant proteins. The analysis is performed in human diploid fibroblasts, TIG-3 cells and their derived cell lines (TS, TSM, and TSR). TIG-3 TS is immortalized cells by expressing hTERT and SV40 largeT and smallT antigens (SV40 ER). TIG3 TSM and TSR cells were transformed lines by cMyc or H-RAS(G12V) in TERT/SV40 expressing TIG-3, respectively.
HostingRepository	jPOST
AnnounceDate	2016-12-03
AnnouncementXML	Submission_2017-08-08_15:52:55.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Masaki Matsumoto
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	mTRAQ light reporter+balance reagent acylated residue; iodoacetamide derivatized residue; mTRAQ:13C(3)15N(1); mTRAQ:13C(6)15N(2)
Instrument	Q TRAP

Revision	Datetime	Status	ChangeLog Entry
0	2016-08-17 21:41:19	ID requested
1	2016-12-02 16:08:38	announced
⏵ 2	2017-08-08 15:52:56	announced	Updated PubMed.
3	2018-09-18 22:26:22	announced	Updated CV, FTP location and Modification list.
4	2021-02-04 17:00:06	announced	2021-02-04: Updated FTP location.
5	2022-09-18 02:50:14	announced	2022-09-18: Updated FTP location.

Matsumoto M, Matsuzaki F, Oshikawa K, Goshima N, Mori M, Kawamura Y, Ogawa K, Fukuda E, Nakatsumi H, Natsume T, Fukui K, Horimoto K, Nagashima T, Funayama R, Nakayama K, Nakayama KI, A large-scale targeted proteomics assay resource based on an in vitro human proteome. Nat Methods, 14(3):251-258(2017) [pubmed]

submitter keyword: metabolism, absolute quantification, MRM

Masaki Matsumoto
lab head
Masaki Matsumoto
contact affiliation	Div. Proteomics, Med. Inst. Bioreg., Kyushu Univ.
dataset submitter

jPOST dataset URI