PXD004762-1

PXD004762 is an original dataset announced via ProteomeXchange.

Title	Identification of substrates of the CRL4-HBx E3 ubiquitin ligase
Description	Hepatitis B Virus (HBV) remains a major public health problem, and is a major cause liver cancer worldwide. HBV infection in vivo requires the function of the HBx protein, which facilitates expression of viral genes from the episomal genome by an unknown mechanism. Evidence suggests that HBx functions as a targeting subunit for the CRL4 E3 ubiquitin ligase, redirecting the complex to target and degrade an unknown host restriction factor. We used substrate trapping proteomics to search for ubiquitylation substrates of HBx. We used MLN4924 to block CRL activity and stabilize interactions between HBx and its substrates. We then performed APMS to identify bound proteins and potential substrates.
HostingRepository	PRIDE
AnnounceDate	2021-03-04
AnnouncementXML	Submission_2021-03-04_12:15:54.054.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christopher Murphy
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-08-11 06:09:01	ID requested
⏵ 1	2021-03-04 12:15:55	announced
2	2024-10-22 04:32:15	announced	2024-10-22: Updated project metadata.

Murphy CM, Xu Y, Li F, Nio K, Reszka-Blanco N, Li X, Wu Y, Yu Y, Xiong Y, Su L, Hepatitis B Virus X Protein Promotes Degradation of SMC5/6 to Enhance HBV Replication. Cell Rep, 16(11):2846-2854(2016) [pubmed]

curator keyword: Biological

submitter keyword: HBx, HBV, Cullin, CRL4, substrate, MLN4924, ubiquitylation

Lishan Su
contact affiliation	Department of Microbiology and Immunology Lineberger Cancer Center University of North Carolina, Chapel Hill
contact email	lishan_su@med.unc.edu
lab head
Christopher Murphy
contact affiliation	UNC
contact email	chrism83@hotmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/09/PXD004762

PRIDE project URI

• PRIDE
  1. PXD004762
     1. Label: PRIDE project
     2. Name: Identification of substrates of the CRL4-HBx E3 ubiquitin ligase