PXD004752 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | iTRAQ-based HepaRG Membrane Proteomics |
Description | HepaRG cell, a stabilized bipotent liver progenitor cell line, exhibits hepatocyte functions only after differentiation. However, the mechanism of transition from non-differentiated to differentiated states, accompanied by proliferation and migration, is poorly understood. Little information exists for proteins involved in this process, particularly those residing in the plasma membrane. In this study, the plasma membrane protein expression change of HepaRG cell before and after differentiation were systematically analyzed using an iTRAQ labeled quantitative membrane proteomics approach. A total of 70 membrane proteins were identified to be differentially expressed. Function and disease clustering analysis showed that 11 of these proteins are involved in migration. Two key factors (MMP-14 and OCLN) were validated by qRT-PCR and Western Blot. Blockade of MMP-14, an extracellular matrix metalloprotease, by monoclonal antibody in a wound healing assay further demonstrated the importance of this protein in tumor cell migration. Even further, the MMP-14 expression correlation with HCC is confirmed by HCC cell lines and tissue samples. |
HostingRepository | PRIDE |
AnnounceDate | 2017-02-08 |
AnnouncementXML | Submission_2017-02-08_06:28:52.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Wei Wei |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iTRAQ4plex-116 reporter+balance reagent acylated residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-08-10 07:11:43 | ID requested | |
⏵ 1 | 2017-02-08 06:28:54 | announced | |
Publication List
Zhao M, Xu F, Wu F, Yu D, Su N, Zhang Y, Cheng L, Xu P, iTRAQ-Based Membrane Proteomics Reveals Plasma Membrane Proteins Change During HepaRG Cell Differentiation. J Proteome Res, 15(12):4245-4257(2016) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: HepaRG, membrane protein, iTRAQ |
Contact List
Ping Xu |
contact affiliation | State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences Beijing, Beijing Institute of Radiation Medicine, Beijing 102206, P. R. China |
contact email | xuping_bprc@126.com |
lab head | |
Wei Wei |
contact affiliation | BPRC |
contact email | powerweiwei@163.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD004752
- Label: PRIDE project
- Name: iTRAQ-based HepaRG Membrane Proteomics