PXD004698-2
PXD004698 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Zinc-responsive labeling in live cells for novel conditional proteomics |
| Description | Zinc signaling and dynamics play significant roles in many physiological responses and diseases. To understand the physiological roles of zinc in detail, comprehensive identification of proteins under high concentration of mobile zinc ion is crucial. In this report, we developed a novel “conditional proteomics” based on a chemical proteomic strategy with designer zinc-responsive labeling reagents (AIZin). In this conditional proteomics, a subset of proteins in a zinc-rich environment is tagged and identified in combination with quantitative mass spectrometry. This method was successfully applied to elucidate zinc dyshomeostasis induced by nitric oxide-triggered oxidative stress in glioma cells and unveiled dynamic changes of the zinc-related proteomes. Moreover, a subset of protein composition identified by our method allowed the characterization of unknown zinc-rich vesicles generated by oxidative stress as endoplasmic reticulum-Golgi-related vesicles. |
| HostingRepository | jPOST |
| AnnounceDate | 2016-08-24 |
| AnnouncementXML | Submission_2017-03-31_00:07:17.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD004698 |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Hironori Achiwa |
| SpeciesList | scientific name: Rattus rattus (Black rat); NCBI TaxID: 10117; |
| ModificationList | iodoacetamide derivatized residue; (2S,3R)-3-hydroxyaspartic acid; TMT6plex-126 reporter+balance reagent acylated residue |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2016-08-01 18:25:18 | ID requested | |
| 1 | 2016-08-24 03:15:03 | announced | |
| ⏵ 2 | 2017-03-31 00:07:18 | announced | Updated DigitalObjectIdentifier. |
| 3 | 2018-09-18 22:46:44 | announced | Updated CV, FTP location and Modification list. |
| 4 | 2021-02-04 17:03:59 | announced | 2021-02-04: Updated FTP location. |
| 5 | 2022-09-18 02:25:05 | announced | 2022-09-18: Updated FTP location. |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: conditional proteomics, chemical proteomics, TMT |
Contact List
| Itaru Hamachi | |
|---|---|
| lab head | |
| Hironori Achiwa | |
| contact affiliation | Kyoto Univ. |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
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