PXD004655 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | SILAC-Tp (12 importins) |
Description | Importin-beta family proteins are nucleocytoplasmic transport receptors (NTRs), and they transport most nuclear proteins as their cargoes into and out of the nuclei. Human cells have 20 species of importin-beta family NTRs, of which 10 (importin-beta, transportin-1, -2, -SR, importin-4, -5, -7, -8, -9, and -11) are nuclear import receptors and 2 (importin-13, and exportin-4) are bi-directional receptors. Here, we identified the import cargo proteins of these 12 NTRs by a method called SILAC-Tp, which employs stable isotope labeling with amino acids in cell culture (SILAC), an in vitro reconstituted transport system, and LC-MS/MS. |
HostingRepository | PRIDE |
AnnounceDate | 2017-01-20 |
AnnouncementXML | Submission_2017-02-02_07:19:56.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Makoto Kimura |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-07-25 23:49:16 | ID requested | |
1 | 2017-01-20 05:03:01 | announced | |
⏵ 2 | 2017-02-02 07:19:58 | announced | Updated publication reference for PubMed record(s): 28117667. |
Publication List
Kimura M, Morinaka Y, Imai K, Kose S, Horton P, Imamoto N, Extensive cargo identification reveals distinct biological roles of the 12 importin pathways. Elife, 6():(2017) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: human, importin, transportin, cargo, nuclear transport, SILAC, LC-MS/MS |
Contact List
Naoko Imamoto |
contact affiliation | Cellular Dynamics Laboratory, RIKEN, Japan |
contact email | nimamoto@riken.jp |
lab head | |
Makoto Kimura |
contact affiliation | RIKEN |
contact email | makimura@riken.jp |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/01/PXD004655 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD004655
- Label: PRIDE project
- Name: SILAC-Tp (12 importins)