PXD004652 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | ULTRADEEP CHARACTERIZATION OF THE DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS INTO MESENCHYMAL STEM CELLS |
Description | Mesenchymal stem cells (MSC) are self-renewing multipotent cells which hold great potential in reconstructive medicine and have been shown to be beneficial for the treatment of a variety of diseases. MSC can be derived from multiple adult tissues but have limited expansion capacity in cell culture. Highly proliferative ESC-derived MSC may be an alternative source for MSC but to date no standardized protocol exists which meets clinical standards. We extended and improved a published protocol (Raynaud et al., 2013) for the differentiation of human embryonic stem cells (ESC) into highly-proliferative MSC. To characterize the differentiation process in-depth we performed comprehensive transcriptome analysis (RNA-seq) combined with large-scale proteomic and phosphoproteomic profiling using quantitative high resolution mass spectrometric analysis. Differentiation was followed over a time course of 30 days including sampling on days 0, 1, 2, 5, 15, and 30. ESC-derived MSC were compared to adult tissue-derived MSC (bone marrow MSC) as well as to their origin (ESC). The established differentiation protocol is highly reproducible and may be adapted for clinical use. The comprehensive analysis of the differentiation process will improve understanding of MSC biology and therefore directly benefit MSC-based therapies. |
HostingRepository | PRIDE |
AnnounceDate | 2019-07-23 |
AnnouncementXML | Submission_2019-07-23_14:40:55.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Johannes Graumann |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-07-25 05:39:19 | ID requested | |
⏵ 1 | 2019-07-23 14:40:56 | announced | |
2 | 2019-07-24 00:30:26 | announced | Updated project metadata. |
3 | 2019-07-26 00:30:17 | announced | Updated project metadata. |
4 | 2019-08-01 04:42:25 | announced | Updated project metadata. |
5 | 2021-10-29 04:35:27 | announced | 2021-10-29: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biomedical |
submitter keyword: Human embryonic stem cells, human mesenchymal stem cells, bone marrow, differentiation, transcriptome, proteome, phosphoproteome, quantitative proteomics, LC-MS/MS, RNA-seq |
Contact List
Johannes Graumann |
contact affiliation | Weill Cornell Medicine - Qatar |
contact email | jog2030@qatar-med.cornell.edu |
lab head | |
Johannes Graumann |
contact affiliation | Weill Cornell Medicine - Qatar |
contact email | jog2030@qatar-med.cornell.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD004652
- Label: PRIDE project
- Name: ULTRADEEP CHARACTERIZATION OF THE DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS INTO MESENCHYMAL STEM CELLS