PXD004637 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Label-free analysis of CD8+ T cell subset proteomes supports a progressive differentiation model of virus-specific T cells in humans |
Description | Different pathogens trigger naïve T cells to express distinct sets of effector proteins. To better understand the molecular mechanisms that drive this functional specification, we used high resolution, label-free mass spectrometry to measure proteomic differences between the seven largest circulating human CD8+ T cell subsets. Unsupervised hierarchical clustering of the proteomes placed naïve and CD45RA-expressing effector-type T cells at the extremes of the spectrum with central-memory and other effector-memory stages located in between. Prominent differences between the subsets included expression of various granzymes, signaling proteins and molecules involved in metabolic regulation. Remarkably, whereas most of the proteomic changes between the subsets were gradual, a small proportion of proteins were regulated only in discrete subsets. The data obtained from this proteome analysis correspond best to a progressive differentiation model in which specific stable traits are gradually acquired during pathogen-specific development. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:31:25.559.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Maartje van den Biggelaar |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion ETD |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-07-21 07:20:57 | ID requested | |
1 | 2017-05-02 01:18:52 | announced | |
2 | 2019-02-12 08:10:16 | announced | Updated publication reference for PubMed record(s): 28467900. |
⏵ 3 | 2024-10-22 04:31:33 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1016/j.celrep.2017.04.014; |
van Aalderen MC, van den Biggelaar M, Remmerswaal EBM, van Alphen FPJ, Meijer AB, Ten Berge IJM, van Lier RAW, T Cell Subset Proteomes Supports a Progressive Differentiation Model of Human-Virus-Specific T Cells. Cell Rep, 19(5):1068-1079(2017) [pubmed] |
Keyword List
curator keyword: Biological, Biomedical |
submitter keyword: Human, CD8+ T cells |
Contact List
Maartje van den Biggelaar |
contact affiliation | Department of Plasma Proteins, Sanquin Research, Amsterdam, the Netherlands |
contact email | m.vandenbiggelaar@sanquin.nl |
lab head | |
Maartje van den Biggelaar |
contact affiliation | Sanquin Research |
contact email | m.vandenbiggelaar@sanquin.nl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/05/PXD004637 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD004637
- Label: PRIDE project
- Name: Label-free analysis of CD8+ T cell subset proteomes supports a progressive differentiation model of virus-specific T cells in humans