PXD004611 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Somatic embryogenesis of Pinus nigra |
Description | There are several issues complicating somatic embryogenesis in conifers, for instance the low initiation frequency, the weak maturation capacity, and the loss of an embryogenic potential after a prolonged cultivation. The aim of our study was to clarify molecular details of the this process in the tree Pinus nigra Arn. We performed comparative proteomic analysis based on the two-dimensional gel electrophoresis in 2 genotypes coded 362 and 366 of: 1) proliferating embryogenic tissues (E) initiated from immature zygotic embryos, 2) non-embryogenic calli (NEC) started from cotyledons of somatic seedlings, 3) embryogenic tissues that lost the maturation capacity (E-L). Pine tissues showed distinct morphologic features: a) E362 and E366 were characterized by the presence of early bipolar structures capable of maturation and plantlet regeneration; b) NEC362 and NEC366 were composed of round shaped cells without any organisation; c) for E-L362 and E-L366 the long-term culture of initially embryogenic tissues resulted in the disorganisation of early bipolar structures. We found 24 differentially accumulated protein spots common for both genotypes after comparison of E versus NEC. |
HostingRepository | PRIDE |
AnnounceDate | 2017-09-06 |
AnnouncementXML | Submission_2017-09-06_07:51:22.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD004611 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Maksym Danchenko |
SpeciesList | scientific name: Pinus nigra; NCBI TaxID: 58042; |
ModificationList | monomethylated residue; phosphorylated residue; deaminated residue; monohydroxylated residue; acetylated residue; monosodium salt; iodoacetamide derivatized residue; deamidated residue |
Instrument | ultrafleXtreme |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-07-20 06:06:28 | ID requested | |
⏵ 1 | 2017-09-06 07:51:23 | announced | |
Publication List
Klubicov, á K, Uv, á, ckov, á L, Danchenko M, Nemecek P, Skult, é, ty L, Salaj J, Salaj T, Insights into the early stage of Pinus nigra Arn. somatic embryogenesis using discovery proteomics. J Proteomics, 169():99-111(2017) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Cell wall biosynthesis |
Conifers |
Embryogenic tissue |
Loss of maturation capacity |
Non-embryogenic callus |
2-D gel electrophoresis |
Contact List
Katarína Klubicová |
contact affiliation | Institute of Plant Genetics and Biotechnology, Plant Science and Biodiversity Center SAS |
contact email | katarina.klubicova@savba.sk |
lab head | |
Maksym Danchenko |
contact affiliation | Institute of Virology, Biomedical Research Center SAS |
contact email | maxymdan@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD004611
- Label: PRIDE project
- Name: Somatic embryogenesis of Pinus nigra