PXD004608-2

PXD004608 is an original dataset announced via ProteomeXchange.

Title	Integrated cellular and plasma proteomics reveals common and systemic signatures in contrasting B-cell cancers
Description	Approximately 800,000 leukaemia and lymphoma cases are diagnosed worldwide each year. Burkitt’s lymphoma and chronic lymphocytic leukaemia represent contrasting examples of B-cell cancers, modelled by Eμ-myc and Eμ-TCL1 mice, respectively. To better understand B-cell cancers and systemic effects of oncogenesis, tumours and plasma from these models were profiled by mass spectrometry proteomics. 8270 cellular and 2095 plasma proteins were fully quantitated by isobaric labelling, of which 695 and 279 demonstrated overabundance coinciding in both tumour models, respectively. Co-occurring upregulated cellular tumour processes included ribosome biogenesis, translation, cell cycle promotion and chromosome segregation. Tumour plasma overabundance highlighted immunity, inflammation, microenvironment interactions, a prolific tumour lysis signature and putative biomarkers of early-stage cancer. Integrative evaluation of tumours and plasma provided systemic insight not captured in isolation. Overall, these findings provide a detailed characterisation of systemic oncogenesis in two contrasting B-cell tumour models, identifying extensive common profiles in both tumour cells and plasma.
HostingRepository	PRIDE
AnnounceDate	2017-10-24
AnnouncementXML	Submission_2017-10-24_04:42:54.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Harvey Johnston
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; methylthiolated residue; phosphorylated residue; iTRAQ8plex-116 reporter+balance reagent acylated residue; monohydroxylated residue; deamidated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2016-07-20 01:37:11	ID requested
1	2017-01-11 05:11:35	announced
⏵ 2	2017-10-24 04:42:55	announced	Updated project metadata.
3	2024-10-22 04:31:30	announced	2024-10-22: Updated project metadata.

Johnston HE, Carter MJ, Cox KL, Dunscombe M, Manousopoulou A, Townsend PA, Garbis SD, Cragg MS, Integrated Cellular and Plasma Proteomics of Contrasting B-cell Cancers Reveals Common, Unique and Systemic Signatures. Mol Cell Proteomics, 16(3):386-406(2017) [pubmed]

curator keyword: Biomedical

submitter keyword: Eµ-myc, Eµ-TCL1, B-cell, oncogenesis, plasma, proteomics, lymphoma, leukaemia

Spiro Dennis Garbis
contact affiliation	Clinical and Experimental Sciences Unit, Faculty of Medicine, University of Southampton, Southampton SO16 6YD, UK Centre for Proteomic Research, Institute for Life Sciences, University of Southampton, Highfield Campus, Southampton, SO17 1BJ, UK
contact email	s.d.garbis@soton.ac.uk
lab head
Harvey Johnston
contact affiliation	University of Southampton
contact email	h.johnston@soton.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD004608
     1. Label: PRIDE project
     2. Name: Integrated cellular and plasma proteomics reveals common and systemic signatures in contrasting B-cell cancers