PXD004592 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A metaproteomic analysis of the response of a freshwater microbial community under nutrient enrichment |
Description | Eutrophication can lead to an uncontrollable increase in algal biomass, which has repercussions for the entire microbial and pelagic community. Studies have shown how nutrient enrichment affects microbial species succession, however details regarding the impact on community functionality are rare. Here, we applied a metaproteomic approach to investigate the functional changes to algal and bacterial communities, over time, in oligotrophic and eutrophic conditions, in freshwater microcosms. Samples were taken early during algal and cyanobacterial dominance and later under bacterial dominance. 1048 proteins, from the two treatments and two timepoints, were identified and quantified by their exponentially modified protein abundance index. In oligotrophic conditions, Bacteroidetes express extracellular hydrolases and Ton-B dependent receptors to degrade and transport high molecular weight compounds captured while attached to the phycosphere. Alpha- and Beta-proteobacteria were found to capture different substrates from algal exudate (carbohydrates and amino acids, respectively) suggesting resource partitioning to avoid direct competition. In eutrophic conditions, environmental adaptation proteins from cyanobacteria suggested better resilience compared to algae in a low carbon nutrient enriched environment. This study provides insight into differences in functional microbial processes between oligo- and eutrophic conditions at different timepoints and highlights how primary producers control bacterial resources in freshwater environments. |
HostingRepository | PRIDE |
AnnounceDate | 2016-08-01 |
AnnouncementXML | Submission_2016-08-01_09:48:14.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD004592 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | David Russo |
SpeciesList | scientific name: mixed sample; NCBI TaxID: 1427524; |
ModificationList | Methylthio; Oxidation |
Instrument | maXis |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-07-18 03:34:19 | ID requested | |
⏵ 1 | 2016-08-01 09:48:15 | announced | |
2 | 2017-06-12 07:30:22 | announced | Updated publication reference for PubMed record(s): 27536273. |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Metaproteomics |
submitter keyword: oligotrophic, eutrophic, metaproteomics, microbial loop, freshwater, LC-MSMS |
Contact List
Jagroop Pandhal |
contact affiliation | University of Sheffield, Faculty of Engineering, Department of Chemical and Biological Engineering, UK |
contact email | j.pandhal@sheffield.ac.uk |
lab head | |
David Russo |
contact affiliation | University of Sheffield |
contact email | davidarusso@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD004592
- Label: PRIDE project
- Name: A metaproteomic analysis of the response of a freshwater microbial community under nutrient enrichment