PXD004590-2

PXD004590 is an original dataset announced via ProteomeXchange.

Title	Characterisation of the human hemostatic O-glycoproteome
Description	The hemostatic system comprises platelet aggregation, coagulation and fibrinolysis, and is critical to the maintenance of vascular integrity. Multiple studies indicate that glycans play an important role in maintaining the hemostatic system, however, most investigations have focused on N-glycans and little is known about the location and function of O-glycans. Here we performed the first systematic analysis of hemostatic O-glycosylation using lectin affinity chromatography coupled to LC-MS/MS to determine the precise location of O-glycans in human plasma, platelets, and endothelial cells. We identified the hitherto largest O-glycoproteome from native tissue, demonstrating that O-glycosylation is a ubiquitous modification of hemostatic proteins.
HostingRepository	PRIDE
AnnounceDate	2017-08-07
AnnouncementXML	Submission_2017-08-06_23:57:27.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sarah King
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; Hex-HexNAc; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2016-07-18 02:51:31	ID requested
1	2017-03-15 06:02:00	announced
⏵ 2	2017-08-06 23:57:28	announced	Updated project metadata.
3	2023-11-14 08:49:10	announced	2023-11-14: Updated project metadata.

https://doi.org/10.1182/bloodadvances.2016002121;

curator keyword: Biomedical

submitter keyword: human, plasma, serum, platelet, endothelial, endothelium, hemostatic, LC-MSMS, orbitrap

Hans Wandall
contact affiliation	University of Copenhagen Faculty Of Health and Medical Sciences Centre for Glycomics Department of Cellular and Molecular Medicine Building 24.6.38 Nørre Alle 20, DK-2200 Copenhagen N Denmark
contact email	hhw@sund.ku.dk
lab head
Sarah King
contact affiliation	Copenhagen University
contact email	sarahki@sund.ku.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/03/PXD004590

PRIDE project URI

• PRIDE
  1. PXD004590
     1. Label: PRIDE project
     2. Name: Characterisation of the human hemostatic O-glycoproteome