PXD004530-2

PXD004530 is an original dataset announced via ProteomeXchange.

Title	Nucleocytoplasmic transport and membrane growth control nuclear size
Description	How cells control the overall size of membrane-bound organelles is an important unanswered question of cell biology. Fission yeast cells maintain a nuclear size that is proportional to cellular size, resulting in a constant ratio between the nuclear and cellular volumes (N/C ratio). To shed light on nuclear size control we conducted a genome-wide visual screen of a fission yeast gene deletion collection for mutants altered in their N/C ratio, and found that defects in both nucleocytoplasmic mRNA transport and nuclear membrane proliferation alter the N/C ratio. Perturbing nuclear mRNA export causes general bulk accumulation of both mRNA and protein, and a N/C ratio increase which is dependent on new membrane synthesis. Dysregulation of nuclear membrane growth also results in an enlarged N/C ratio, and additionally generates an aberrant nuclear shape. We propose that both properly regulated nucleocytoplasmic transport and nuclear membrane growth are central for controlling nuclear size.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:30:53.799.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andrew Jones
SpeciesList	scientific name: Schizosaccharomyces pombe; NCBI TaxID: 4896;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-07-08 03:17:44	ID requested
1	2017-06-05 06:17:51	announced
⏵ 2	2024-10-22 04:31:02	announced	2024-10-22: Updated project metadata.

Kume K, Cantwell H, Neumann FR, Jones AW, Snijders AP, Nurse P, A systematic genomic screen implicates nucleocytoplasmic transport and membrane growth in nuclear size control. PLoS Genet, 13(5):e1006767(2017) [pubmed]

10.1371/journal.pgen.1006767;

curator keyword: Biological

submitter keyword: Nuclear Cell Ratio, Nuclear Size,SILAC

Ambrosius Snijders
contact affiliation	Protein Analysis and Proteomics The Francis Crick Institute Mass Spectrometry STP
contact email	Bram.Snijders@crick.ac.uk
lab head
Andrew Jones
contact affiliation	The Francis Crick Institute
contact email	Andrew.Jones@crick.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/06/PXD004530

PRIDE project URI

• PRIDE
  1. PXD004530
     1. Label: PRIDE project
     2. Name: Nucleocytoplasmic transport and membrane growth control nuclear size