PXD004452-1

PXD004452 is an original dataset announced via ProteomeXchange.

Title	HeLa proteome of 12,250 protein-coding genes
Description	We developed an optimized multi-shot proteomics workflow based on high-resolution offline high pH reversed-phase peptide separation of high peptide loads collecting many fractions that were in turn analyzed by short online chromatographic separations and fast peptide sequencing using orbitrap tandem mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2017-06-12
AnnouncementXML	Submission_2017-06-12_07:29:12.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christian Kelstrup
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monomethylated residue; phosphorylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-06-28 01:56:01	ID requested
⏵ 1	2017-06-12 07:29:13	announced
2	2024-10-22 04:30:42	announced	2024-10-22: Updated project metadata.

Bekker-Jensen DB, Kelstrup CD, Batth TS, Larsen SC, Haldrup C, Bramsen JB, S, ø, rensen KD, H, ø, yer S, Ø, rntoft TF, Andersen CL, Nielsen ML, Olsen JV, An Optimized Shotgun Strategy for the Rapid Generation of Comprehensive Human Proteomes. Cell Syst, 4(6):587-599.e4(2017) [pubmed]

curator keyword: Technical, Biological

submitter keyword: shotgun proteomics, deep proteomes, hela, cell lines, tissue

Jesper V. Olsen
contact affiliation	Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, Denmark
contact email	jesper.olsen@cpr.ku.dk
lab head
Christian Kelstrup
contact affiliation	University of Copenhagen
contact email	chdake@cpr.ku.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/06/PXD004452

PRIDE project URI

• PRIDE
  1. PXD004452
     1. Label: PRIDE project
     2. Name: HeLa proteome of 12,250 protein-coding genes