PXD004451 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Dendritic cells infected with Salmonella enterica |
Description | Salmonella enterica causes serious global burden of morbidity and mortality and is a major cause of infant bacteremia in sub Saharan Africa. Diseases caused by Salmonella are treatable with antibiotics but successful antibiotic treatment has become difficult due to antimicrobial resistance. An effective vaccine together with public health effort may therefore be a better strategy to control these infections. Protective immunity against Salmonella depends primarily on T cell-mediated immune responses and therefore identifying relevant T cell antigens is necessary for Salmonella vaccine development. Our laboratory has used an immunoproteomics approach to identify Chlamydia T cell antigens that exhibited significant protection against Chlamydia infection in mice. In this study, we infected murine bone marrow derived dendritic cells from C57BL/6 mice with Salmonella enterica strain SL1344 followed by isolation of MHC class I and II- molecules and elution of bound peptides. The sequences of the peptides were then identified using tandem mass spectrometry. We identified 87 MHC class II and 23 MHC class I Salmonella derived peptides. Four of 12 peptides stimulated IFN-? production by CD4 T cells from the spleens of mice with persistent Salmonella infection. These antigens will be useful for Salmonella immunobiology research and are potential Salmonella vaccine candidates. |
HostingRepository | PRIDE |
AnnounceDate | 2016-12-21 |
AnnouncementXML | Submission_2016-12-21_04:50:40.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Queenie Chan |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-06-28 01:51:30 | ID requested | |
⏵ 1 | 2016-12-21 04:50:41 | announced | |
2 | 2017-10-24 03:05:35 | announced | Updated project metadata. |
Publication List
Karunakaran KP, Yu H, Jiang X, Chan Q, Goldberg MF, Jenkins MK, Foster LJ, Brunham RC, Identification of MHC-Bound Peptides from Dendritic Cells Infected with Salmonella enterica Strain SL1344: Implications for a Nontyphoidal Salmonella Vaccine. J Proteome Res, 16(1):298-306(2017) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: typhoid fever, vaccine development, antigens |
Contact List
Leonard J Foster |
contact affiliation | University of British Columbia, Department of Biochemistry, Michael Smith Laboratories |
contact email | foster@chibi.ubc.ca |
lab head | |
Queenie Chan |
contact affiliation | Centre for High Throughput Biology |
contact email | queeniecwt@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD004451
- Label: PRIDE project
- Name: Dendritic cells infected with Salmonella enterica