PXD004404-1

PXD004404 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteome analysis of hepatocellular carcinoma tissue
Description	To identify potential markers for early HCC diagnosis we performed a label-free proteome analysis using samples collected from 19 patients. We analyzed the data considering events known to take place in early events of HCC development such as abnormal regulation of Wnt/b-catenin and activation of receptor tyrosine kinases (RTKs). We expected that such analysis can lead to the identification of potential biomarkers for early-stage HCC diagnosis. 31 proteins functionally linked to downstream interactors of Wnt/b-catenin and parts of RTK's-activated pathways Ras and JAK/STAT were selected for verification in a larger and independent cohort (n= 31) using selected (multiple) reaction monitoring (SRM/MRM).
HostingRepository	PeptideAtlas
AnnounceDate	2016-06-21
AnnouncementXML	Submission_2016-06-21_10:41:28.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Wael Naboulsi
SpeciesList
ModificationList	6x(13)C,2x(15)N labeled L-lysine; 6x(13)C,4x(15)N labeled L-arginine
Instrument	6490 Triple Quadrupole LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2016-06-21 08:30:02	ID requested
⏵ 1	2016-06-21 10:41:29	announced

Naboulsi W, Megger DA, Bracht T, Kohl M, Turewicz M, Eisenacher M, Voss DM, Schlaak JF, Hoffmann AC, Weber F, Baba HA, Meyer HE, Sitek B, Quantitative Tissue Proteomics Analysis Reveals Versican as Potential Biomarker for Early-Stage Hepatocellular Carcinoma. J Proteome Res, 15(1):38-47(2016) [pubmed]

curator keyword: selected reaction monitoring, SRM, targeted

Wael Naboulsi
contact affiliation	Ruhr-Universität Bochum
contact email	Wael.Naboulsi@rub.de
dataset submitter
Barbara Sitek
contact affiliation	Ruhr-Universität Bochum
contact email	barbara.sitek@rub.de
lab head

PeptideAtlas dataset URI

PASSEL experiment URI

PASSEL transition group browser URI