PXD004394-1
PXD004394 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Integrated Quantification method for precise and robust protein measurement in clinical proteomics, study 1 |
| Description | Background: Current quantification methods for mass spectrometry (MS)-based proteomics either do not provide sufficient control of variability or are difficult to implement for routine clinical testing. Results: We present here an integrated quantification (InteQuan) method that better controls pre-analytical and analytical variability than the popular quantification method using stable isotope-labeled standard peptides (SISQuan). We quantified 16 lung cancer biomarker candidates in human plasma samples in three assessment studies, using immunoaffinity depletion coupled with multiple reaction monitoring (MRM) MS. InteQuan outperformed SISQuan in precision in all three studies and tolerated a two-fold difference in sample loading. The three studies lasted over six months and encountered major changes in experimental settings. Nevertheless, plasma proteins in low ng/ml to low μg/ml concentrations were measured with a median technical coefficient of variation (CV) of 11.9% using InteQuan. The corresponding median CV using SISQuan was 15.3% after linear fitting. Furthermore, InteQuan surpassed SISQuan in measuring biological difference among clinical samples and in distinguishing benign versus cancer plasma samples. Conclusions: We demonstrated that InteQuan is a simple yet robust quantification method for MS-based quantitative proteomics, especially for applications in biomarker research and in routine clinical testing. |
| HostingRepository | PeptideAtlas |
| AnnounceDate | 2016-06-20 |
| AnnouncementXML | Submission_2016-06-20_16:24:12.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Lik Wee Lee |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | 6x(13)C,4x(15)N labeled L-arginine; 6x(13)C,2x(15)N labeled L-lysine |
| Instrument | 6490 Triple Quadrupole LC/MS |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2016-06-20 16:20:28 | ID requested | |
| ⏵ 1 | 2016-06-20 16:24:14 | announced |
Publication List
| Li XJ, Lee LW, Hayward C, Brusniak MY, Fong PY, McLean M, Mulligan J, Spicer D, Fang KC, Hunsucker SW, Kearney P, An integrated quantification method to increase the precision, robustness, and resolution of protein measurement in human plasma samples. Clin Proteomics, 12(1):3(2015) [pubmed] |
Keyword List
| curator keyword: selected reaction monitoring, SRM, targeted |
Contact List
| Lik Wee Lee | |
|---|---|
| contact affiliation | Integrated Diagnostics |
| contact email | llee@indidx.com |
| dataset submitter | |
| Paul Kearney | |
| contact affiliation | Integrated Diagnostics |
| contact email | pkearney@indidx.com |
| lab head | |
Full Dataset Link List
| PeptideAtlas dataset URI |
| PASSEL experiment URI |
| PASSEL transition group browser URI |
| PASSEL transition group browser URI |
| PeptideAtlas dataset URI |
| PASSEL transition group browser URI |
| PeptideAtlas dataset URI |
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