PXD004375-2
PXD004375 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Human 18 FDA approved plasma proteins |
| Description | BACKGROUND: The development of commercially available panels for human blood plasma screening via selected reaction monitoring (SRM) offers reliable, cost-efficient and highly-standardized discovery and validation of protein biomarkers. However, protein detection by SRM can be hampered by interfering peptide fragment ions. To estimate the influence of interference on protein detection, we performed different types of sample preparation and implemented SRM measurements for well-characterized protein targets approved by the US Food and Drug Administration. METHODS: We used the PlasmaDeepDiveâ SRM assay from BiognoSYS AG for absolute quantification of 18 proteins in 19 samples of human plasma using three different protocols for sample preparation. SRM measurements were performed using iRT standards for retention time normalization and isotopically-labeled reference peptides for absolute quantification. SpectroDiveâ software was used for automated detection of reliable peak groups. RESULTS: Fourteen targeted proteins were quantitatively measured in more than half of the samples. Depletion of highly-abundant plasma proteins and peptide fraction clean-up on centrifuge plates resulted in detection of all 18 targeted proteins in femtomolar to picomolar concentrations. CONCLUSIONS: It was shown that commercially designed SRM kits are suitable for SRM detection of well-established plasma/serum biomarkers. |
| HostingRepository | PeptideAtlas |
| AnnounceDate | 2016-06-20 |
| AnnouncementXML | Submission_2016-06-20_14:29:41.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Olga Kiseleva |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | 6x(13)C,4x(15)N labeled L-arginine; 6x(13)C,2x(15)N labeled L-lysine; S-carboxamidomethyl-L-cysteine |
| Instrument | TSQ Vantage |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2016-06-20 13:57:42 | ID requested | |
| 1 | 2016-06-20 14:26:19 | announced | |
| ⏵ 2 | 2016-06-20 14:29:42 | announced | update title |
Publication List
| Kiseleva OI, Romashova YA, Moskaleva NE, Petushkova NA, Teryaeva NB, Belyaev AY, Lisitsa AV, Plasma preparation to measure FDA-approved protein markers by selected reaction monitoring. Clin Transl Med, 4(1):32(2015) [pubmed] |
Keyword List
| curator keyword: selected reaction monitoring, SRM, targeted |
Contact List
| Olga Kiseleva | |
|---|---|
| contact affiliation | Institute of Biomedical Chemistry, Moscow |
| contact email | olly.kiseleva@gmail.com |
| dataset submitter | |
| Lisitsa A.V. | |
| contact affiliation | Institute of Biomedical Chemistry, Moscow |
| contact email | lisitsa063@gmail.com |
| lab head | |
Full Dataset Link List
| PeptideAtlas dataset URI |
| PASSEL experiment URI |
| PASSEL transition group browser URI |
| PASSEL transition group browser URI |
| PeptideAtlas dataset URI |
| PASSEL transition group browser URI |
| PeptideAtlas dataset URI |
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