PXD004304 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Analysis of mixed-oxysterol treatment on the THP-1 macrophage |
Description | THP-1 monocytes were differentiated, via PMA treatment, to macrophages. THP-1 macrophages were then treated with a mixed-oxysterol treatment (7-ketocholesterol and 7beta-hydroxycholesterol) for 24 hours. The mixed-oxysterol treatment was mixed to a ratio that represents the cytotoxic oxysterol content of human atherosclerotic plaque. Control and treated macrophages were analysed using both 2-DE with peptide mass-fingerprinting and tandem-mass spectrometry. |
HostingRepository | PRIDE |
AnnounceDate | 2017-03-15 |
AnnouncementXML | Submission_2017-04-03_02:14:44.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Liam Ward |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap Velos; Voyager-DE PRO |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-06-10 02:53:08 | ID requested | |
1 | 2017-03-15 08:23:54 | announced | |
⏵ 2 | 2017-04-03 02:14:46 | announced | Updated publication reference for PubMed record(s): 28350877. |
Publication List
Ward LJ, Ljunggren SA, Karlsson H, Li W, Yuan XM, Exposure to atheroma-relevant 7-oxysterols causes proteomic alterations in cell death, cellular longevity, and lipid metabolism in THP-1 macrophages. PLoS One, 12(3):e0174475(2017) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: THP-1, macrophage, oxysterol, proteomics, 2-DE, tandem-mass spectrometry |
Contact List
Xi-Ming Yuan |
contact affiliation | Occupational and Environmental Medicine Center, and Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden. |
contact email | ximing.yuan@liu.se |
lab head | |
Liam Ward |
contact affiliation | Division of Clinical Sciences, Department of Clinical and Experimental Medicine, Linköping University. |
contact email | liam.ward@liu.se |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/03/PXD004304 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD004304
- Label: PRIDE project
- Name: Analysis of mixed-oxysterol treatment on the THP-1 macrophage