PXD004285 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Non-intein splicing of RB47 |
Description | The poly A binding protein RB47 was purified from C. reinhardtii chloroplasts and was found to have endoribonuclease activity. MS analysis suggested that an internal non-conserved linker (NCL)sequence was absent from the native protein. Recombinant, full length RB47 protein, including the NCL did not have endoribonuclease activity while versions lacking the NCL were active. An in vitro system, utilizing recombinant RB47 and chloroplast lysates, recreated the processing of RB47 in that the NCL was excised and the flanking sequences were spliced together. |
HostingRepository | PRIDE |
AnnounceDate | 2018-10-27 |
AnnouncementXML | Submission_2018-10-26_16:10:08.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Stephen Campbell |
SpeciesList | scientific name: Chlamydomonas reinhardtii; NCBI TaxID: 3055; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-06-07 01:19:11 | ID requested | |
⏵ 1 | 2018-10-26 16:10:09 | announced | |
Publication List
Campbell SJ, Stern DB, Activation of an Endoribonuclease by Non-intein Protein Splicing. J Biol Chem, 291(31):15911-15922(2016) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Intein, RB47, C. reinhardti, chloroplast |
Contact List
David Stern |
contact affiliation | Boyce Thompson Institute |
contact email | ds28@cornell.edu |
lab head | |
Stephen Campbell |
contact affiliation | Boyce Thompson Institute |
contact email | sjc53@cornell.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD004285 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD004285
- Label: PRIDE project
- Name: Non-intein splicing of RB47