PXD004275-1

PXD004275 is an original dataset announced via ProteomeXchange.

Title	Combination of multi-enzyme digestions and C-TAILS approach for deep screening of C-terminome
Description	Protein C-termini study is still a challenging task and far behind its counterpart N-termini study. The identification of C-termini is often hampered by low ionization response in MS and fewer efficient enrichment methods existing. We previously optimized the C-TAILS (C-terminal amine-based isotope labeling of substrates) method to achieve 75% more identification of protein C-terminal peptides. Although with the significant improvement, the performance of method is not comparable with a regular proteome study since the identification number is very limited. Herein we reported an optimized method by introduction of multiple-enzyme digestions in order to increase the capacity and application of C-TAILS in biological and clinical study. To allow the application of other enzymes than Arg-C in C-TAILS method, we evaluated the effect of the omission of prior amine protection at protein level and revealed the negligible effect caused by. Subsequently we applied five different enzymes in the C-TAILS and evaluated the different performances on the basis of identification and complementariness. As a result, 722 protein C-termini of E. coli were identified in a triplicate experiments using 50 μg each, and the favored enzyme and enzyme combination were discovered and discussed.
HostingRepository	PRIDE
AnnounceDate	2017-11-24
AnnouncementXML	Submission_2017-11-24_04:57:18.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	yang zhang
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	acetylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2016-06-03 03:23:06	ID requested
⏵ 1	2017-11-24 04:57:19	announced
2	2024-10-22 04:29:52	announced	2024-10-22: Updated project metadata.

Zhang Y, Li Q, Huang J, Wu Z, Huang J, Huang L, Li Y, Ye J, Zhang X, An Approach to Incorporate Multi-Enzyme Digestion into C-TAILS for C-Terminomics Studies. Proteomics, 18(1):(2018) [pubmed]

curator keyword: Technical

submitter keyword: Protein C-termini, C-TAILS, multi-enzyme, SCII,

Xumin Zhang
contact affiliation	State Key Laboratory of Genetic Engineering, Department of Biochemistry, School of Life Sciences, Fudan University, Shanghai 200438, China
contact email	xumin_zhang@fudan.edu.cn
lab head
yang zhang
contact affiliation	State Key Laboratory of Genetic Engineering, Department of Biochemistry, School of Life Sciences, Fudan University, Shanghai 200438, China
contact email	wdyangzh@hotmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD004275
     1. Label: PRIDE project
     2. Name: Combination of multi-enzyme digestions and C-TAILS approach for deep screening of C-terminome