PXD004261-1

PXD004261 is an original dataset announced via ProteomeXchange.

Title	Protein expression and phosphoproteome of brown adipose tissue in the hibernating ground squirrel
Description	Mammalian hibernation is characterized by metabolic rate depression and a strong decrease in core body temperature that together create energy savings such that most species do not have to eat over the winter months. Brown adipose tissue (BAT), a thermogenic tissue that uses uncoupled mitochondrial respiration to generate heat instead of ATP, plays a major role in rewarming from deep torpor. The present study used label-free phosphoproteomics to investigate changes in BAT from thirteen-lined ground squirrels (Ictidomys tridecemlineatus), comparing euthermic squirrels with squirrels in deep torpor. Differential expression of mitochondrial proteins was also investigated. Surprisingly, mitochondrial membrane and matrix protein expression in BAT was largely constant between active euthermic squirrels and their hibernating counterparts. Validation by immunoblotting confirmed that the protein levels of mitochondrial respiratory chain complexes were largely unchanged. However, phosphoproteomics showed that pyruvate dehydrogenase (PDH) phosphorylation increased during ground squirrel hibernation, and this was confirmed by immunoblotting with phospho-specific antibodies. PDH phosphorylation leads to its inactivation, which suggests that BAT carbohydrate oxidation is inhibited during hibernation. Phosphorylation of hormone-sensitive lipase (HSL) also increased during hibernation, suggesting that HSL would be in a partly activated state in BAT to produce the fatty acids that are likely the primary fuel for thermogenesis during arousal.
HostingRepository	PRIDE
AnnounceDate	2017-07-11
AnnouncementXML	Submission_2017-07-11_02:31:24.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Didier Vertommen
SpeciesList	scientific name: Ictidomys tridecemlineatus; NCBI TaxID: 43179;
ModificationList	phosphorylated residue; monohydroxylated residue
Instrument	LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2016-06-01 01:58:33	ID requested
⏵ 1	2017-07-11 02:31:25	announced
2	2017-07-13 05:20:14	announced	Updated publication reference for PubMed record(s): 28698229.

Dataset with its publication pending

submitter keyword: metabolic depression, protein phosphorylation, mitochondrial protein expression, lipolysis

Mark H. Rider
contact affiliation	de Duve Institute, Université Catholique de Louvain, Avenue Hippocrate 75, B-1200 Brussels, Belgium
contact email	mark.rider@uclouvain.be
lab head
Didier Vertommen
contact affiliation	UCL - de Duve Institute, Brussels Belgium
contact email	didier.vertommen@uclouvain.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/07/PXD004261

PRIDE project URI

• PRIDE
  1. PXD004261
     1. Label: PRIDE project
     2. Name: Protein expression and phosphoproteome of brown adipose tissue in the hibernating ground squirrel