PXD004248-2

PXD004248 is an original dataset announced via ProteomeXchange.

Title	A Global Analysis of Receptor Tyrosine Kinase – Protein Phosphatase Interactome
Description	Receptor tyrosine kinases (RTKs) and protein phosphatases comprise protein families that play crucial roles in cell signaling. However, a comprehensive picture of how RTKs functionally associate with phosphatases remains elusive. We aimed to study this problem by using two protein-protein interaction (PPI) approaches, the Membrane Yeast Two-Hybrid (MYTH) and the Mammalian Membrane Two-Hybrid (MaMTH), to map the PPIs between 48 human RTKs and 126 phosphatases. The resulting RTK-phosphatase interactome reveals a considerable number of novel interactions and suggests specific roles for different phosphatase families. Additionally, the differential PPIs of some protein tyrosine phosphatases (PTP) and their mutants suggest diverse mechanisms of these PTPs in the regulation of RTK signaling. We further investigated the biological functions of several interactors, and found that PTPRH and PTPRB directly dephosphorylate EGFR and repress its downstream signaling. In contrast, PTPRA plays a dual role in EGFR signaling since besides dephosphorylating EGFR, it conversely enhances downstream ERK signaling through activating SRC. We present the first comprehensive RTK-phosphatase interactome study, providing a broad and deep view of RTK signaling.
HostingRepository	PRIDE
AnnounceDate	2017-10-24
AnnouncementXML	Submission_2017-10-24_04:44:28.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hongbo Guo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-05-31 02:07:41	ID requested
1	2017-01-11 03:28:21	announced
⏵ 2	2017-10-24 04:44:29	announced	Updated project metadata.
3	2024-10-22 04:29:57	announced	2024-10-22: Updated project metadata.

Yao Z, Darowski K, St-Denis N, Wong V, Offensperger F, Villedieu A, Amin S, Malty R, Aoki H, Guo H, Xu Y, Iorio C, Kotlyar M, Emili A, Jurisica I, Neel BG, Babu M, Gingras AC, Stagljar I, A Global Analysis of the Receptor Tyrosine Kinase-Protein Phosphatase Interactome. Mol Cell, 65(2):347-360(2017) [pubmed]

curator keyword: Biological

submitter keyword: Tyrosine Kinaase, phosphatase interactome

Igor Stagljar
contact affiliation	Donnelly Centre, Department of Molecular Genetics,Department of Biochemistry,University of Toronto, Ontario, Canada
contact email	igor.stagljar@utoronto.ca
lab head
Hongbo Guo
contact affiliation	University of Toronto
contact email	hongboguo88@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/01/PXD004248

PRIDE project URI

• PRIDE
  1. PXD004248
     1. Label: PRIDE project
     2. Name: A Global Analysis of Receptor Tyrosine Kinase – Protein Phosphatase Interactome