PXD004236 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | In vivo protein interaction network analysis reveals porin-localized toxin inactivation in antibiotic resistant bacteria |
Description | The nosocomial pathogen Acinetobacter baumannii is a frequent cause of hospital acquired infections worldwide, and a challenge for treatment due to its evolved resistance to antibiotics, including carbapenems. To gain insight on A. baumannii antibiotic resistance mechanisms, we analyzed the protein interaction network of a multidrug-resistant A. baumannii clinical strain Ab5075. Using in vivo chemical cross-linking and mass spectrometry, we identified 2,068 non-redundant cross-linked peptide pairs containing 245 intra- and 398 inter- molecular interactions. Outer membrane proteins OmpA and YiaD, and carbapenemase Oxa-23 are hubs of the identified interaction network. Eighteen novel interactors of Oxa-23 were identified. Interactions of Oxa-23 with outer membrane porins OmpA and CarO were verified with co-immunoprecipitation analysis. Furthermore, transposon mutagenesis of oxa-23 or interactors of Oxa-23 demonstrated changes in meropenem or imipenem sensitivity in Ab5075. These results provide the first view of a porin-localized toxin inactivation model and increase understanding of bacterial antibiotic resistance mechanisms. |
HostingRepository | PRIDE |
AnnounceDate | 2017-10-24 |
AnnouncementXML | Submission_2017-10-24_03:17:21.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Xia Wu |
SpeciesList | scientific name: Acinetobacter baumannii AB5075; NCBI TaxID: 1116234; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ FT |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-05-27 01:56:42 | ID requested | |
1 | 2016-12-22 07:31:34 | announced | |
⏵ 2 | 2017-10-24 03:17:23 | announced | Updated project metadata. |
Publication List
Wu X, Chavez JD, Schweppe DK, Zheng C, Weisbrod CR, Eng JK, Murali A, Lee SA, Ramage E, Gallagher LA, Kulasekara HD, Edrozo ME, Kamischke CN, Brittnacher MJ, Miller SI, Singh PK, Manoil C, Bruce JE, In vivo protein interaction network analysis reveals porin-localized antibiotic inactivation in Acinetobacter baumannii strain AB5075. Nat Commun, 7():13414(2016) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Acinetobacter baumannii, antibiotic resistance, carbapenem, chemical cross-linking, protein-protein interactions |
Contact List
James E. Bruce |
contact affiliation | Department of Genome Sciences, University of Washington, Seattle, USA |
contact email | jimbruce@uw.edu |
lab head | |
Xia Wu |
contact affiliation | University of Washington |
contact email | xiawu2@uw.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD004236
- Label: PRIDE project
- Name: In vivo protein interaction network analysis reveals porin-localized toxin inactivation in antibiotic resistant bacteria