PXD004219-3

PXD004219 is an original dataset announced via ProteomeXchange.

Title	C4PR_LIV Identification of protein biomarkers for pneumococcal meningitis in children
Description	Bacterial meningitis is usually fatal without treatment. Prompt and accurate diagnosis coupled with the timely administration of parenteral antibiotics is necessary in order to save lives. We used label-free mass spectrometry based quantitative proteomics to identify specific protein signatures in cerebrospinal fluid from Malawian children with Streptococcus pneumoniae infection, the leading cause of bacterial meningitis in children, elderly and people infected with Human Immunodeficiency Virus. Samples were analysed in two independent cohorts (cases and controls, n=12 for the discovery cohort and n=16 for the verification cohort). The protein profiles clearly discriminated cases and controls. Proteins involved in the immune response and exosome cell signaling were significantly enriched in the infected samples. Several S. pneumoniae membrane proteins were identified suggesting these as potential therapeutic targets. For both cohorts, over 200 human proteins were up and down-regulated in children with confirmed S. pneumoniae infection in comparison with controls. Compiling proteins up- and down- regulated in both discovery and verification cohorts, we generated a comprehensive list of over 140 proteins from which diagnostic biomarkers could be nominated. For a panel of five selected proteins (cathelicidin, ceruloplasmin, myeloperoxidase, cystatin C and protein S100A8/S100 A9), we additionally verified the label-free results by automated quantitative western blot immunoassays (Simple Western™), with excellent agreement between the two experimental approaches. The proteins identified in our study could form the basis of development of a biomarker panel for rapid diagnosis. Our results could lead to the development of point-of-care assays to inform diagnosis, and to nominate new targets for drugs and vaccines to improve disease management and consequently to enhance the prognosis of children with bacterial meningitis.
HostingRepository	PRIDE
AnnounceDate	2017-08-04
AnnouncementXML	Submission_2017-08-04_00:30:25.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD004219
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Guadalupe Gomez Baena
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Oxidation; Carbamidomethyl
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-05-24 09:47:05	ID requested
1	2017-08-02 11:19:42	announced
2	2017-08-03 02:37:01	announced	Updated project metadata.
⏵ 3	2017-08-04 00:30:26	announced	Updated project metadata.
4	2017-08-07 07:31:32	announced	Updated project metadata.
5	2019-08-06 00:30:19	announced	Updated project metadata.

Dataset with its publication pending

curator keyword: Biological, Biomedical

submitter keyword: bacterial meningitis, Streptococcus pneumoniae, protein quantification

Prof. Rob J. Beynon
contact affiliation	Centre for Proteome Research, University of Liverpool
contact email	rbeynon@liverpool.ac.uk
lab head
Guadalupe Gomez Baena
contact affiliation	University of Liverpool
contact email	g.gomez-baena@liverpool.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD004219
     1. Label: PRIDE project
     2. Name: C4PR_LIV Identification of protein biomarkers for pneumococcal meningitis in children