PXD004208 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Different mechanisms of biotransformation of bisphenol A by the biphenyl-degrading Cupriavidus basilensis SBUG 290 lead to detoxification and reduction of estrogenicity |
Description | A bacterial strain identified as Cupriavidus basilensis uses aromatic compounds as carbon and energy sources and has a high capability to transform the structurally related and hormonally active substance bisphenol A (BPA). Biphenyl-grown and phenol-grown cells converted BPA to five products within 24 h of incubation representing four different transformation pathways: (a) ring hydroxylation, (b) ring fission, (c) transamination and acetylation, and (d) dimerization. Products of the ring fission pathway were non-toxic and all five products exhibited a significantly reduced estrogenic activity compared to BPA. Cell cultivation in nutrient broth resulted in lower product quantities and dimerization was not proved. Thus the question arose whether enzymes of the biphenyl or phenol degradation pathway are involved in the transformation of BPA. Proteomic analyses revealed the constitutive expression of biphenyl degrading enzymes and indicated that the 2,3 dihydroxybiphenyl-1,2-dioxygenase might catalyse the meta-cleavage of the aromatic ring of BPA while enzymes of other pathways seemed to be involved in ring hydroxylation. |
HostingRepository | PRIDE |
AnnounceDate | 2018-07-19 |
AnnouncementXML | Submission_2018-07-19_05:33:42.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Daniela Zuehlke |
SpeciesList | scientific name: Cupriavidus basilensis; NCBI TaxID: 68895; |
ModificationList | monohydroxylated residue |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-05-23 07:14:29 | ID requested | |
⏵ 1 | 2018-07-19 05:33:44 | announced | |
Publication List
Z, ü, hlke MK, Schl, ü, ter R, Mikolasch A, Z, ü, hlke D, Giersberg M, Schindler H, Henning AK, Frenzel H, Hammer E, Lalk M, Bornscheuer UT, Riedel K, Kunze G, Schauer F, Biotransformation and reduction of estrogenicity of bisphenol A by the biphenyl-degrading Cupriavidus basilensis. Appl Microbiol Biotechnol, 101(9):3743-3758(2017) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: bisphenol A-degradation, LC-MS/MS |
Contact List
Katharina Riedel |
contact affiliation | Institute for Microbiology University of Greifswald Friedrich-Ludwig-Jahnstr. 15 17489 Greifswald Germany |
contact email | riedela@uni-greifswald.de |
lab head | |
Daniela Zuehlke |
contact affiliation | Ernst Moritz Arndt University Greifswald, Institute of Microbiology |
contact email | zuehlke@uni-greifswald.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD004208
- Label: PRIDE project
- Name: Different mechanisms of biotransformation of bisphenol A by the biphenyl-degrading Cupriavidus basilensis SBUG 290 lead to detoxification and reduction of estrogenicity