PXD004195-2

PXD004195 is an original dataset announced via ProteomeXchange.

Title	Proteomic and phosphoproteomic profiling of ErbB2-dependent signalling
Description	The mechanisms of ErbB2 signalling and the effects of its overexpression are not fully understood. Herein, SILAC expression profiling and phosphopeptide enrichment of a relevant, non-transformed, immortalized human mammary luminal epithelial cell model were used to profile ErbB2-dependent differences in protein expression and phosphorylation events triggered via EGFR (EGF treatment) and ErbB3 (HRGbeta1 treatment) in the context of ErbB2 overexpression. Bioinformatics analysis was used to infer changes in cellular processes and signalling events. We demonstrate the complexity of the responses to oncogene expression and growth factor signalling and identify protein changes relevant to ErbB2-dependent altered cellular phenotype, in particular cell cycle progression and hyper-proliferation, reduced adhesion and enhanced motility. Numerous novel sites of growth factor-regulated phosphorylation were also identified that were enhanced by ErbB2 overexpression and we putatively link these to altered cell behaviour. Moreover, we have defined a novel mechanism by which ErbB signalling suppresses basal interferon signalling that would promote the survival and proliferation of mammary luminal epithelial cells.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:29:52.693.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	John Timms
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	carbamoylated residue; phosphorylated residue; 6x(13)C labeled residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2016-05-23 01:47:33	ID requested
1	2018-10-26 07:16:38	announced
⏵ 2	2024-10-22 04:29:57	announced	2024-10-22: Updated project metadata.

Worthington J, Spain G, Timms JF, Effects of ErbB2 Overexpression on the Proteome and ErbB Ligand-specific Phosphosignaling in Mammary Luminal Epithelial Cells. Mol Cell Proteomics, 16(4):608-621(2017) [pubmed]

10.1074/mcp.M116.061267;

curator keyword: Biological

submitter keyword: ErbB2

breast cancer

LC-MS/MS

SILAC

phosphoproteomics

John Francis Timms
contact affiliation	University College London
contact email	john.timms@ucl.ac.uk
lab head
John Timms
contact affiliation	University College London
contact email	john.timms@ucl.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD004195

PRIDE project URI

• PRIDE
  1. PXD004195
     1. Label: PRIDE project
     2. Name: Proteomic and phosphoproteomic profiling of ErbB2-dependent signalling