PXD004190-1

PXD004190 is an original dataset announced via ProteomeXchange.

Title	Quantitative GTPase affinity purification identifies Rho family protein interaction partners
Description	Although Rho GTPases are essential molecular switches involved in many cellular processes, an unbiased experimental comparison of their interaction partners was not yet performed. Here, we develop quantitative GTPase affinity purification (qGAP) to systematically identify interaction partners of six Rho GTPases (Cdc42, Rac1, RhoA, RhoB, RhoC, RhoD) depending on their nucleotide loading state. We use Stable isotope labeling by Amino acids in cell culture (SILAC) and label free quantification (LFQ). Our interaction network contains many new proteins, reveals highly promiscuous binding of several effectors and mirrors evolutionary relationships of Rho GTPases.
HostingRepository	PRIDE
AnnounceDate	2016-12-23
AnnouncementXML	Submission_2016-12-23_15:06:19.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Florian Paul
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive; LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2016-05-19 05:43:07	ID requested
⏵ 1	2016-12-23 15:06:20	announced
2	2017-10-24 06:12:18	announced	Updated project metadata.
3	2024-10-22 04:30:15	announced	2024-10-22: Updated project metadata.

Paul F, Zauber H, von Berg L, Rocks O, Daumke O, Selbach M, Quantitative GTPase Affinity Purification Identifies Rho Family Protein Interaction Partners. Mol Cell Proteomics, 16(1):73-85(2017) [pubmed]

submitter keyword: Rho GTPase, interaction proteomics

Dr Matthias Selbach
contact affiliation	Max-Delbrück-Centrum für Molekulare Medizin (MDC) Robert-Rössle-Str. 10 13092 Berlin
contact email	matthias.selbach@mdc-berlin.de
lab head
Florian Paul
contact affiliation	Max Delbrück Center
contact email	florianpaul@gmx.net
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/12/PXD004190

PRIDE project URI

• PRIDE
  1. PXD004190
     1. Label: PRIDE project
     2. Name: Quantitative GTPase affinity purification identifies Rho family protein interaction partners