PXD004182-2

PXD004182 is an original dataset announced via ProteomeXchange.

Title	HCD fragmentation of glycated peptides
Description	Protein glycation is a highly disease-relevant concentration-dependent non-enzymatic reaction of reducing sugars with amine groups of proteins to form early as well as advanced glycation products (AGEs). To complement our blood proteomics studies in diabetics, we here established higher-energy collisional dissociation (HCD) fragmentation on Orbitrap mass spectrometers for analyzing protein glycation. We evaluated parameters to most efficiently fragment and identify early glycation products on in-vitro glycated model proteins. We then applied our optimized workflow for glycation analysis of the entire HeLa proteome as well as for single-run analysis of undepleted and unenriched blood plasma and whole blood. We conclude that HCD fragmentation is well suited for analyzing glycated peptides when integrating the dominant neutral loss into the analysis and that single-run plasma proteomics measurements have great potential to diagnosing the diabetic status of patients.
HostingRepository	PRIDE
AnnounceDate	2019-12-16
AnnouncementXML	Submission_2019-12-16_06:43:11.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mario Oroshi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	glycated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-05-18 09:00:07	ID requested
1	2016-07-25 08:32:14	announced
⏵ 2	2019-12-16 06:43:12	announced	2019-12-16: Updated project metadata.
3	2024-10-22 04:33:38	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

curator keyword: Technical, Biological

submitter keyword: Protein glycation, higher-energy collisional dissociation, diabetes, blood plasma, AGEs

Matthias Mann
contact affiliation	Department Proteomics and Signal Transduction Max Planck Institute of Biochemistry Am Klopferspitz 18 D-82152 Martinsried
contact email	mmann@biochem.mpg.de
lab head
Mario Oroshi
contact affiliation	Proteomics
contact email	oroshi@biochem.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/01/PXD004182

PRIDE project URI

• PRIDE
  1. PXD004182
     1. Label: PRIDE project
     2. Name: HCD fragmentation of glycated peptides