PXD004179-2

PXD004179 is an original dataset announced via ProteomeXchange.

Title	Sulfolobus islandicus PTM and quantitative study using bottom-up proteomics
Description	Protein posttranslational methylation and acetylation have been reported to occur in archaea, including the genus Sulfolobus, but have never been characterized on a proteome-wide scale. Among important Sulfolobus proteins carrying such modifications are the chromatin proteins that have been described to be methylated and acetylated on lysine side chains, resembling eukaryotic histones in that aspect. To get more insight into the extent of these modifications and their dynamics during the different growth steps of the thermoacidophylic archaeon S. islandicus , we performed a global and deep proteomics analysis using a combination of high-throughput bottom-up and top-down proteomics approaches on a single high-resolution mass spectrometer. 1,931 methylation sites on 751 proteins were found by the bottom-up analysis, with methylation sites of 424-526 proteins monitored throughout three cell culture growth stages: Early, Mid and Late. The previously described aKMT4 methyltransferase was found to be potentially responsible for this massive modification.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:29:19.562.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Egor Vorontsov
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Sulfolobus islandicus LAL14/1; NCBI TaxID: 1241935;
ModificationList	monomethylated residue; acetylated residue
Instrument	Orbitrap Fusion ETD

Revision	Datetime	Status	ChangeLog Entry
0	2016-05-18 05:02:52	ID requested
1	2016-09-13 04:49:42	announced
⏵ 2	2024-10-22 04:29:20	announced	2024-10-22: Updated project metadata.

Vorontsov EA, Rensen E, Prangishvili D, Krupovic M, Chamot-Rooke J, Abundant Lysine Methylation and N-Terminal Acetylation in Sulfolobus islandicus Revealed by Bottom-Up and Top-Down Proteomics. Mol Cell Proteomics, 15(11):3388-3404(2016) [pubmed]

submitter keyword: methylation,Archaea, Sulfolobus islandicus, label-free quantitation, Orbitrap Fusion, posttranslational modification

Julia Chamot-Rooke
contact affiliation	Unité de Spectrométrie de Masse Structurale et Protéomique, Institut Pasteur, 28 rue du Dr. Roux, 75015 Paris, France
contact email	julia.chamot-rooke@pasteur.fr
lab head
Egor Vorontsov
contact affiliation	Proteomics Core Facility, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
contact email	yegor.msu@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/09/PXD004179

PRIDE project URI

• PRIDE
  1. PXD004179
     1. Label: PRIDE project
     2. Name: Sulfolobus islandicus PTM and quantitative study using bottom-up proteomics