PXD004133 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Mycobacterium tuberculosis protein production pipeline |
Description | Purpose: Tuberculosis is still a major threat to global health. New tools and strategies to produce disease-related proteins are quintessential for the development of novel vaccines and diagnostic markers. Experimental design: To obtain recombinant proteins from Mycobacterium tuberculosis (Mtb) for use in clinical applications, a standardized procedure was developed that includes subcloning, protein expression in Mycobacterium smegmatis and protein purification using chromatography. The potential for the different protein targets to serve as diagnostic markers for tuberculosis was established using multiplex immunoassays. Results: Twelve soluble proteins from Mtb, including one protein complex, were purified to homogeneity following recombinant expression in M. smegmatis. Protein purity was assessed both by size exclusion chromatography and mass spectrometry. Multiplex serological testing of the final protein preparations showed that all but one protein displayed a clear antibody response in serum samples from 278 tuberculosis patients. Conclusion and clinical relevance: The established workflow comprises a simple, cost-effective and scalable pipeline for production of soluble proteins from Mtb and can be used to prioritize immunogenic proteins suitable for use as diagnostic markers. |
HostingRepository | PRIDE |
AnnounceDate | 2018-06-27 |
AnnouncementXML | Submission_2018-06-27_03:51:29.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Joanna Kirkpatrick |
SpeciesList | scientific name: Mycobacterium tuberculosis H37Rv; NCBI TaxID: 83332; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-05-11 05:45:54 | ID requested | |
⏵ 1 | 2018-06-27 03:51:30 | announced | |
Publication List
Milewski MC, Broger T, Kirkpatrick J, Filomena A, Komadina D, Schneiderhan-Marra N, Wilmanns M, Parret AH, A standardized production pipeline for high profile targets from Mycobacterium tuberculosis. Proteomics Clin Appl, 10(9-10):1049-1057(2016) [pubmed] |
Keyword List
curator keyword: Biological, Biomedical |
submitter keyword: Mycobacterium tuberculosis, protein production, diagnostic marker |
Contact List
Annabel Parret |
contact affiliation | Head of the Molecular Biology and Biochemistry Laboratory EMBL Hamburg Notkestrasse 85, 22607 Hamburg, Germany |
contact email | parret@embl-hamburg.de |
lab head | |
Joanna Kirkpatrick |
contact affiliation | Leibniz Institute on Aging - FLI |
contact email | joanna.kirkpatrick@leibniz-fli.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
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- PRIDE
- PXD004133
- Label: PRIDE project
- Name: Mycobacterium tuberculosis protein production pipeline