PXD004118-2

PXD004118 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomic analysis of two different rice varieties reveals that drought tolerance is correlated with reduced abundance of photosynthetic machinery and increased abundance of ClpD1 protease
Description	Rice is the major staple food for more than half of world's population. As global climate changes, we are observing more floods, droughts and severe heat waves. Two rice cultivars with contrasting genetic backgrounds and levels of tolerance to drought, Nipponbare and IAC1131, were used in this study. Four-week-old seedlings of both cultivars were grown in large soil volumes and then exposed to moderate and extreme drought for 7 days, followed by 3 days of re-watering. Mature leaves were harvested from plants from each treatment for protein extraction and subsequent shotgun proteomic analysis, with validation of selected proteins by western blotting. Gene Ontology (GO) annotations of differentially expressed proteins provide insights into the metabolic pathways that are involved in drought stress resistance. Our data indicate that IAC1131 appears to be better able to cope with stressful conditions by up regulating a suite of stress and defence response related proteins. Nipponbare, in contrast, lacks the range of stress responses shown by the more stress tolerant variety, and responds to drought stress by initiating a partial shutdown of chlorophyll biosynthesis in an apparent attempt to preserve resources.
HostingRepository	PRIDE
AnnounceDate	2018-01-31
AnnouncementXML	Submission_2019-01-21_03:16:51.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yunqi Wu
SpeciesList	scientific name: Rice(O. sativa); NCBI TaxID: 4530;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-05-09 04:04:02	ID requested
1	2018-01-31 00:20:28	announced
⏵ 2	2019-01-21 03:16:53	announced	Updated publication reference for PubMed record(s): 27195813, 30656201.
3	2024-10-22 04:30:25	announced	2024-10-22: Updated project metadata.

Wu Y, Mirzaei M, Atwell BJ, Haynes PA, Label-free and isobaric tandem mass tag (TMT) multiplexed quantitative proteomic data of two contrasting rice cultivars exposed to drought stress and recovery. Data Brief, 22():697-702(2019) [pubmed]

Wu Y, Mirzaei M, Pascovici D, Chick JM, Atwell BJ, Haynes PA, Quantitative proteomic analysis of two different rice varieties reveals that drought tolerance is correlated with reduced abundance of photosynthetic machinery and increased abundance of ClpD1 protease. J Proteomics, 143():73-82(2016) [pubmed]

curator keyword: Biological

submitter keyword: Abiotic stress / Drought tolerance / Plant proteomics / Rice / Shotgun proteomics / ClpD1 protease

Paul A. Haynes
contact affiliation	Department of Chemistry and Biomolecular Sciences, Faculty of Science and Engineering, Macquarie University
contact email	paul.haynes@mq.edu.au
lab head
Yunqi Wu
contact affiliation	Macquarie University
contact email	yunqi.wu@mq.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD004118
     1. Label: PRIDE project
     2. Name: Quantitative proteomic analysis of two different rice varieties reveals that drought tolerance is correlated with reduced abundance of photosynthetic machinery and increased abundance of ClpD1 protease