PXD004117-2

PXD004117 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of the extracellular and intracellular role of IL-33 in primary human endothelial cells
Description	IL-33 is a nuclear cytokine from the IL-1 family that plays important roles in health and disease.Under healthy conditions, IL-33 is constitutively expressed to high levels in the nucleus of producing cells in various human and mouse tissues. The extracellular function of IL-33 cytokine has been well documented, but it remains unclear whether intracellular nuclear IL-33 has additional functions in the nucleus. Here, we used a global proteomic approach based on quantification of 5000 individual proteins by high-resolution mass spectrometry to compare the extracellular and intracellular roles of IL-33 in primary human endothelial cells, a major source of IL-33 protein in human tissues. Large-scale analysis of protein expression was performed either after stimulation of the cells with the IL-33 mature form IL-3395-270 (during 6h or 24h) or after siRNA knockdown of intracellular IL-33 (two experiments, each with a different pool of distinct siRNAs, noted siRNA1 and siRNA2). In each case, proteins were fractionated by 1D SDS-PAGE in 12 gel bands, and label-free quantitative analysis was performed. The present dataset corresponds to the stimulation of human endothelial cells with IL-3395-270 during 6h (IL33_6) or 24h (IL33_24). Cells cultured in the absence of IL-33 were used as control (CTil33). Three independent biological replicates (noted _A, _B, _C) were prepared and analyzed for each condition, leading to 9 different samples. Each of them was fractionated into 12 gel bands analyzed by nanoLC-MS/MS, leading to 108 raw files.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:29:26.346.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Anne Gonzalez de Peredo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-05-09 02:05:45	ID requested
1	2016-10-10 02:44:41	announced
⏵ 2	2024-10-22 04:29:27	announced	2024-10-22: Updated project metadata.

Gautier V, Cayrol C, Farache D, Roga S, Monsarrat B, Burlet-Schiltz O, Gonzalez de Peredo A, Girard JP, Extracellular IL-33 cytokine, but not endogenous nuclear IL-33, regulates protein expression in endothelial cells. Sci Rep, 6():34255(2016) [pubmed]

10.1038/srep34255;

curator keyword: Biological

submitter keyword: cytokine, inflammation, endothelial cells, human, fractionation, shotgun,IL-33, HUVEC

Anne Gonzalez de Peredo
contact affiliation	Institute of Pharmacology and Structural Biology, CNRS, Toulouse, France
contact email	gonzalez@ipbs.fr
lab head
Anne Gonzalez de Peredo
contact affiliation	CNRS/IPBS
contact email	gonzalez@ipbs.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD004117
     1. Label: PRIDE project
     2. Name: Proteomic analysis of the extracellular and intracellular role of IL-33 in primary human endothelial cells